Genomics

Dataset Information

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Transcriptome analysis of rhesus monkey germinal vesicle and failed-to-mature oocytes


ABSTRACT: Oocyte maturation defects are one contributing factor in unexplained female infertility. Failure of some oocytes to undergo germinal vesicle breakdown or progress to second meiotic metaphase in response to an ovulatory stimulus can limit the number of high quality oocytes available for assisted reproduction. Understanding the molecular mechanisms that underlie oocyte maturation failure may lead to novel methods for increasing the number of high quality oocytes available for assisted reproduction, as well as providing new insight into the basic biology of oocyte maturation. Using RNA sequencing, we compared the transcriptome of rhesus monkey oocytes that failed to mature (FTM) in response to an ovulatory stimulus in vivo to normal germinal vesicle and metaphase II stage oocytes. Overall design: Transcriptomes of rhesus monkey oocytes that failed to mature (FTM) in response to an ovulatory stimulus in vivo, normal germinal vesicle, and metaphase II stage oocytes were compared using RNAseq.

INSTRUMENT(S): Illumina HiSeq 4000 (Macaca mulatta)

SUBMITTER: Uros Midic  

PROVIDER: GSE112533 | GEO | 2018-09-06

REPOSITORIES: GEO

Dataset's files

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Action DRS
GSE112533_GV_FTM.counts.txt.gz Txt
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Publications

Transcriptome analysis of rhesus monkey failed-to-mature oocytes: deficiencies in transcriptional regulation and cytoplasmic maturation of the oocyte mRNA population.

Ruebel Meghan L ML   Schall Peter Z PZ   Midic Uros U   Vincent Kailey A KA   Goheen Benjamin B   VandeVoort Catherine A CA   Latham Keith E KE  

Molecular human reproduction 20181001 10


<h4>Study question</h4>Which different pathways and functions are altered in rhesus monkey oocytes that fail to mature after an ovulatory stimulus?<h4>Summary answer</h4>Failed to mature (FTM) oocytes complete a large portion of the transition in transcriptome composition associated with normal maturation, but also manifest numerous differences that indicate incomplete transcriptional repression and cytoplasmic maturation affecting multiple processes.<h4>What is known already</h4>Oocyte maturati  ...[more]

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