Dataset Information

Transcriptome analysis of rhesus monkey germinal vesicle and failed-to-mature oocytes

ABSTRACT: Oocyte maturation defects are one contributing factor in unexplained female infertility. Failure of some oocytes to undergo germinal vesicle breakdown or progress to second meiotic metaphase in response to an ovulatory stimulus can limit the number of high quality oocytes available for assisted reproduction. Understanding the molecular mechanisms that underlie oocyte maturation failure may lead to novel methods for increasing the number of high quality oocytes available for assisted reproduction, as well as providing new insight into the basic biology of oocyte maturation. Using RNA sequencing, we compared the transcriptome of rhesus monkey oocytes that failed to mature (FTM) in response to an ovulatory stimulus in vivo to normal germinal vesicle and metaphase II stage oocytes. Overall design: Transcriptomes of rhesus monkey oocytes that failed to mature (FTM) in response to an ovulatory stimulus in vivo, normal germinal vesicle, and metaphase II stage oocytes were compared using RNAseq.

INSTRUMENT(S): Illumina HiSeq 4000 (Macaca mulatta)

SUBMITTER: Uros Midic

PROVIDER: GSE112533 | GEO | 2018-09-06

REPOSITORIES: GEO

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Dataset's files

Source:

			Action	DRS
	GSE112533_GV_FTM.counts.txt.gz	Txt

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Publications

Transcriptome analysis of rhesus monkey failed-to-mature oocytes: deficiencies in transcriptional regulation and cytoplasmic maturation of the oocyte mRNA population.

Ruebel Meghan L ML Schall Peter Z PZ Midic Uros U Vincent Kailey A KA Goheen Benjamin B VandeVoort Catherine A CA Latham Keith E KE

Molecular human reproduction 20181001 10

<h4>Study question</h4>Which different pathways and functions are altered in rhesus monkey oocytes that fail to mature after an ovulatory stimulus?<h4>Summary answer</h4>Failed to mature (FTM) oocytes complete a large portion of the transition in transcriptome composition associated with normal maturation, but also manifest numerous differences that indicate incomplete transcriptional repression and cytoplasmic maturation affecting multiple processes.<h4>What is known already</h4>Oocyte maturati ...[more]

PMID: 30085220

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Project description:BACKGROUND:Cumulus cells (CC) encapsulate growing oocytes and support their growth and development. Transcriptomic signatures of CC have the potential to serve as valuable non-invasive biomarkers for oocyte competency and potential. The present sibling cumulus-oocyte-complex (COC) cohort study aimed at defining functional variations between oocytes of different maturity exposed to the same stimulation conditions, by assessing the transcriptomic signatures of their corresponding CC. CC were collected from 18 patients with both germinal vesicle and metaphase II oocytes from the same cycle to keep the biological variability between samples to a minimum. RNA sequencing, differential expression, pathway analysis, and leading-edge were performed to highlight functional differences between CC encapsulating oocytes of different maturity. RESULTS:Transcriptomic signatures representing CC encapsulating oocytes of different maturity clustered separately on principal component analysis with 1818 genes differentially expressed. CCs encapsulating mature oocytes were more transcriptionally synchronized when compared with CCs encapsulating immature oocytes. Moreover, the transcriptional activity was lower, albeit not absent, in CC encapsulating mature oocytes, with 2407 fewer transcripts detected than in CC encapsulating immature (germinal vesicle - GV) oocytes. Hallmark pathways and ovarian processes that were affected by oocyte maturity included cell cycle regulation, steroid metabolism, apoptosis, extracellular matrix remodeling, and inflammation. CONCLUSIONS:Herein we review our findings and discuss how they align with previous literature addressing transcriptomic signatures of oocyte maturation. Our findings support the available literature and enhance it with several genes and pathways, which have not been previously implicated in promoting human oocyte maturation. This study lays the ground for future functional studies that can enhance our understanding of human oocyte maturation.