Positive emotion-specific changes in gene expression profile in hypothalamus of the tickled rats
Ontology highlight
ABSTRACT:
ORGANISM(S): Rattus norvegicus
PROVIDER: GSE11267 | GEO | 2013/08/02
SECONDARY ACCESSION(S): PRJNA106667
REPOSITORIES: GEO
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Project description:The changes of mRNA expression levels were analyzed in striatum, salivary glands , and peripheral leukocyte of the juvenile rat stimulated by tickling for 4 weeks as a positive stimulation. Keywords: Stress response Twenty-one-day-old male Wistar ratsM-cM-^@M-^@after weaning were used. All rats were separately maintained in the standard cages which were shielded with screens not to see each other during the study. The rats were then subjected to the following three conditions; tickling, light touch, and no stimulus. The stimulation was given to the rats once every weekday (Monday to Friday) for 4 weeks. Tickling stimulation was used as a mimic of the rough-and-tumble play of rats which induced a positive emotional response [50 kHz ultrasonic vocalization (USV)]. Light touch was a discriminative stimulation to tickling, and neither the rat with light touch nor with no stimulus showed such a response in general. Gene expression changes in striatum, salivary glands, and peripheral leukocyte of the tickled rats were comprehensively analyzed comparing with light touched and no stimulated rats using microarray techniques. Biological replicates: 4 animals each condition. One replicate per array.
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Project description:The changes of mRNA expression levels were analyzed in striatum, salivary glands , and peripheral leukocyte of the juvenile rat stimulated by tickling for 4 weeks as a positive stimulation. Keywords: Stress response
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Project description:By using a 44k chicken Agilent microarray, we systematically analyzed the chicken hypothalamus transcriptome response to thermal stress. Twelve hypothalamus samples were chosen from three groups (four samples per group) to be used in chicken genome microarray to examine differential gene expression.We compared the expression profiles between each pairs of the three groups using the microarray data. Totally, 2474 genes were found to be differentially expressed in the three comparisons with p＜0.05 and fold change (FC) higher than 1.5 and the genes were mainly involved in self-regulation and compensation required to maintain homeostasis, including heat shock protein family, enzyme and the hormone, neurotransmitter, cell-cell signaling, metabolism and cytokines. The transcripts of heat shock protein including Hsp 40 and Hsp 90 were significantly changed respond to thermal stress and genes involved in regulation of cell morphogenesis were significantly upregulated in heat stressed group with comparison to control and temperature recovery group. Additionally, the down-regulated genes in both heat stress and temperature recovery groups compared to control group were enriched in muscle organ development, striated muscle tissue development, cardiac muscle tissue development and muscle tissue development, which indicates that muscle development was inhibited during and in short-term after heat treatment. Most of genes dysregulated in heat stress group were found to be recovered in temperature recovery group, which confirmed their roles they could play in coping with heat stress. The present study provides a broader understanding of molecular mechanisms underlying the stress response in chicken and discovery of novel genes that are regulated in a thermal stress specific manner.
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