Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

LH3 overexpression reverses profound alterations of gene transcriptional profile in cerebral vessels in hypertension-induced spontaneous intracerebral hemorrhage

ABSTRACT: To gain mechanistic insights into the protection effects of lysyl hydroxylase (LH3) on reducing the susceptibility to intracerebral hemorrhage(ICH), a whole transcriptomic analysis of cerebral vessels were performed using RNA-seq. It is noteworthy that our preliminary data demonstrated that mice did not developed ICH until day 7 after AngII plus L-NAME infusion, cerebral vessels for RNA-seq were acquired at day 5 in order to provide the mechanistic or causal evidence of a direct participatory role of LH3 to the protection effects of ICH.

ORGANISM(S): Mus musculus

PROVIDER: GSE113151 | GEO | 2018/04/16

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Identification of inflammation-related microRNAs deregulated in the plasma from intracerebral hemorrhage patients

Project description:We tested the hypothesis that circulating microRNAs (miRNAs) present in plasma might display a specific signature in patients with intracerebral hemorrhage (ICH). Global miRNA profiles were determined with the Agilent Human miRNA Microarray platform, 027233. ICH patients display a characteristic inflammation-related miRNA profile as compared to healthy controls. Plasma samples were collected from the following 6 subject groups: male ICH patients (n=8), female ICH patients (n=7), male healthy control (n=4), female healthy control (n=4), male ischemic stroke patients (n=8) and female ischemic stroke patients (n=8). Total RNAs isolated from 1 ml plasma were pooled for each group. A fixed volume of RNA sample was withdrawn from each pool and used for microarray detection.

2013-01-19 | E-GEOD-43618 | biostudies-arrayexpress

Leukocyte dynamics after intracerebral hemorrhage in a living patient reveal rapid adaptations to tissue milieu

Project description:Intracerebral hemorrhage (ICH) is a devastating form of stroke with a high mortality rate and few treatment options. Discovery of therapeutic interventions has been slow given the challenges associated with studying acute injury, particularly over time, in the human brain. Inflammation induced by exposure of brain tissue to blood appears to be a major part of brain tissue injury. Here we longitudinally profiled blood and cerebral hematoma effluent from a patient enrolled in the Minimally Invasive Surgery with Thrombolysis in Intracerebral Haemorrhage Evacuation (MISTIEIII) trial, offering a rare window into the local and systemic immune responses to acute brain injury. Using single-cell RNA-sequencing, we characterized the local cellular response during ICH in the brain of a living patient at single-cell resolution for the first time. Our analysis revealed rapid shifts in the activation states of myeloid and T cells in the brain over time, suggesting that leukocyte responses are dynamically reshaped by the hematoma microenvironment. Interestingly, the patient had an asymptomatic re-bleed (second local exposure to blood) that our transcriptional data indicated occurred more than 30 hours prior to detection by CT scan. This case highlights the rapid immune dynamics in the brain after ICH and suggests that sensitive methods like scRNA-seq can inform our understanding of complex intracerebral events.

2021-06-02 | GSE166638 | GEO

Genome-wide DNA methylation pattern in whole blood associated with primary intracerebral hemorrhage

Project description:To reveal the role of DNA methylation in peripheral blood from primary intracerebral hemorrhage(ICH) ,DNAs from 30 ICH patients and 34 matched controls were analyzed by methylation microarray. In total, we identified 1377 hypomethylated and 153 hypermethylated differentially methylated positions (DMPs) in peripheral blood from ICH patients compared to healthy controls.

2021-07-10 | GSE179759 | GEO

LH3 overexpression reverses profound alterations of gene transcriptional profile in cerebral vessels in hypertension-induced spontaneous intracerebral hemorrhage

Project description:LH3 overexpression reverses profound alterations of gene transcriptional profile in cerebral vessels in hypertension-induced spontaneous intracerebral hemorrhage

| PRJNA450267 | ENA

Analysis of Age-Dependent Transcriptomic Changes in Response to Intracerebral Hemorrhage in Mice

Project description:To investigate age-dependent transcriptomic changes between young or aged intracerebral hemorrhage mice, we established collagenase IV-induced intracerebral hemorrhage mice models. Intracerebral hemorrhage was induced by infusion of sterile collagenase IV in ipsilateral caudate putamen of brain. We then performed gene expression profiling analysis using data obtained from RNA-seq of brain perihematomal tissues from young or aged ICH mice 24 hours after intracerebral hemorrhage.

2022-04-29 | GSE200575 | GEO

Development of gene expression signatures in experimental intracerebral hemorrhage

Project description:To identify the potential lncRNA and mRNA post intracerebral hemorrhage (ICH), we have employed lncRNA microarray expression profiling. 570 mRNAs and 313 lncRNAs were identified in the ICH vs sham group .Expression of six mRNA (Saa3, Col10a1, Mmp13, Oxt, Pcdhb6 and Resp18) and lncRNA (ENSMUT00000141700, AK143011, ENSMUT00000128306, AK030988, ENSMUST00000187076 and ENSMUST00000134129) from this signature was quantified by qPCR.

2023-05-17 | GSE227683 | GEO

Identification of inflammation-related microRNAs deregulated in the plasma from intracerebral hemorrhage patients

2013-01-19 | GSE43618 | GEO

Effect of Pterostilbene (PTE) treatment on gene expression after intracerebral hemorrhage injury

Project description:To investigate the effect of Pterostilbene(PTE) on gene expression after intracerebral hemorrhage injury, we employed RNA sequencing (RNA-seq) to profile the mRNA expression ensuing PTE treatment after ICH. Different gene distribution could be clearly seen on the volcano plot between diferent comparisions among Sham, ICH, and ICH+PTE group. Finally, we showed that 1021 shared genes overlapping between those differentially expressed genes, which was reversed by PTE treatment

2024-03-01 | GSE228222 | GEO

Effect of P7C3-A20 treatment on gene expression after intracerebral hemorrhage injury

Project description:To investigate the effect of P7C3-A20 on gene expression after intracerebral hemorrhage injury, we employed RNA sequencing (RNA-seq) to profile the mRNA expression ensuing P7C3-A20 treatment after ICH. Different gene distribution could be clearly seen on the volcano plot between diferent comparisions among Sham, ICH, and ICH+P7C3-A20 group. Finally, we showed that 1047 shared genes overlapping between those differentially expressed genes, which was reversed by P7C3-A20 treatment

2022-11-15 | GSE216607 | GEO

TMT-based quantitative proteomics reveals the protective mechanism of Tenuigenin after experimental intracerebral hemorrhage in mice

Project description:Tenuigenin (TNG), an extract obtained from Polygalae Radix, possesses anti-inflammatory,anti-oxidant, neuro-protective properties. However, the potential mechanism of TNG on intracerebral hemorrhage (ICH) has not been well studied. Therefore, in this study, we aimed to identify the prospective mechanism of TNG in treating ICH with the help of TMT-based quantitative proteomics. Our results showed that the optimal dose of TNG was 16mg/kg, which could markedly improve neurological functions, reduce cerebral edema, hematoma volume and hemoglobin level 72h after ICH. Furthermore,our proteomic results identified that a total of 404 DEPs (353 up and 51 down regulated) were identified in ICH+vehicle vs Sham group, while 342 DEPs (306 up and 36 down regulated) and 76 DEPs (28 up and 48 down regulated) were quantified in TNG vs Sham group and TNG vs ICH+vehicle group respectively. In addition, a total of 26 DEPs were selected out according to strict criteria. Complement and coagulation cascades was the most significantly enriched pathway and two proteins (MBL-C and Car1) were further validated as hub molecules.

2023-09-15 | PXD045270 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data