Dataset Information


Genomic DNA sequencing of budding yeast strains

ABSTRACT: We report DNA content of yeast strains that are growing asynchronously or treated with hydroxyurea (HU). We selected two strains from our Epi-Decoder library, that are wild-type but have BAR1 or RPO21 TAP-tagged. This reveals that HU-treated samples have peaks in DNA content representing stalled replication forks. Overall design: Whole-genome DNA sequencing of two biological replicates with and without hydroxyurea

INSTRUMENT(S): Illumina HiSeq 2000 (Saccharomyces cerevisiae)

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Tessy Korthout  

PROVIDER: GSE114290 | GEO | 2018-07-16


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Decoding the chromatin proteome of a single genomic locus by DNA sequencing.

Korthout Tessy T   Poramba-Liyanage Deepani W DW   van Kruijsbergen Ila I   Verzijlbergen Kitty F KF   van Gemert Frank P A FPA   van Welsem Tibor T   van Leeuwen Fred F  

PLoS biology 20180713 7

Transcription, replication, and repair involve interactions of specific genomic loci with many different proteins. How these interactions are orchestrated at any given location and under changing cellular conditions is largely unknown because systematically measuring protein-DNA interactions at a specific locus in the genome is challenging. To address this problem, we developed Epi-Decoder, a Tag-chromatin immunoprecipitation-Barcode-Sequencing (TAG-ChIP-Barcode-Seq) technology in budding yeast.  ...[more]

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