Genomics

Dataset Information

0

Regulation of Translation Elongation Revealed by Ribosome Profiling [Dataset_4]


ABSTRACT: Ribosomes undergo substantial conformational changes during translation elongation to accommodate incoming aminoacyl-tRNAs and translocate along the mRNA template. We used multiple elongation inhibitors and chemical probing to define ribosome conformational states corresponding to different sized ribosome-protected mRNA fragments (RPFs) generated by ribosome profiling. We show using various genetic and environmental perturbations that the previously identified 20-22 nucleotide (nt) RPFs correspond predominantly to ribosomes in a pre-accommodation state with an open 40S ribosomal A site while the classical 27-29 nt fragments correspond to ribosomes in a pre-translocation state with an occupied 40S ribosomal A site. These distinct ribosome conformational states revealed by ribosome profiling are seen in all eukaryotes tested including fungi, worms and mammals. This high-resolution ribosome profiling approach reveals the anticipated Rck2-dependent inhibition of translocation through eEF2 phosphorylation during hyperosmotic stress. These same approaches reveal a strong translation elongation arrest during oxidative stress where the ribosomes are trapped in a pre-translocation state, but in this case the translational arrest is independent of Rck2-driven eEF2 phosphorylation. These results provide new insights and approaches for defining the molecular events that impact translation elongation throughout biology.

ORGANISM(S): Homo sapiens Saccharomyces cerevisiae Caenorhabditis elegans

PROVIDER: GSE115161 | GEO | 2019/01/27

REPOSITORIES: GEO

Similar Datasets

2019-01-27 | GSE115160 | GEO
2019-01-27 | GSE115159 | GEO
2019-01-27 | GSE115158 | GEO
2017-08-16 | GSE98333 | GEO
2011-04-30 | BIOMD0000000457 | BioModels
2020-10-28 | GSE160206 | GEO
2018-05-14 | GSE106448 | GEO
| E-GEOD-58321 | biostudies-arrayexpress
2020-06-15 | GSE149697 | GEO
2020-06-01 | GSE131074 | GEO