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Genetic signatures of retinal ganglion cells revealed through single cell profiling [dissociated RGC]

ABSTRACT: This study aimed to characterize the transcriptomes of adult retinal ganglion cells (RGCs). These cells were isolated through their expression of a TdTomato molecule that would only turn on when Cre was present. Cre was controlled by the Parvalbumin promoter.

ORGANISM(S): Mus musculus

PROVIDER: GSE115332 | GEO | 2019/11/01

REPOSITORIES: GEO

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Genetic signatures of retinal ganglion cells revealed through single cell profiling [non-dissociated RGC]

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Retinal ganglion cells lipid profiling

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Inducible Rbpms-CreERT2 mouse line for functional studies of genes in retinal ganglion cell physiology and disease

Project description:Retinal ganglion cells (RGCs) are the sole output neurons conveying visual stimuli from the retina to the brain and the dysfunction or loss of RGCs is the primary determinant of visual loss in traumatic and degenerative ocular conditions. Currently, there is a lack of RGC-specific Cre mouse lines that serve as invaluable tools for manipulating genes in RGCs and studying the genetic basis of RGC diseases. The RNA-binding protein with multiple splicing (RBPMS) is identified as the specific marker of all RGCs. Here, we report the generation and characterization of a knock-in mouse line in which a P2A-CreERT2 coding sequence is fused in-frame to the C-terminus of endogenous RBPMS, allowing the co-expression of RBPMS and CreERT2. The inducible Rbpms-CreERT2 mice exhibited a high recombination efficiency in activating the expression of tdTomato reporter gene in nearly all adult RGCs as well as in differentiated RGCs starting at E13.5. Additionally, heterozygous Rbpms-CreERT2 knock-in mice showed no detectable defect in the retinal structure, visual function and transcriptome. Altogether, our results demonstrated that the Rbpms-CreERT2/+ knock-in mouse can serve as a powerful and highly desired genetic tool for lineage tracing, genetic manipulation, retinal physiology study and ocular disease modeling in RGCs.

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Transcription profiling of chicken whole eye and retinal ganglion cells (RGC) to identify RGC-specific genes

Project description:Identification of genes regulated by ATH5 in a dose-dependent manner.; Identification of retinal ganglion cells (RGC)-specific genes.

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Single cell RNA sequencing of stem cell-derived retinal ganglion cells

Project description:We used human embryonic stem cell-derived retinal ganglion cells (RGCs) to characterize the transcriptome of 1,174 cells at the single cell level. The human embryonic stem cell line BRN3B-mCherry A81-H7 was differentiated to RGCs using a guided differentiation approach. Cells were harvested at day 36 and incubated with THY1 antibody (Miltenyi) before undergoing FACS. THY1 positive and THY1 negative cells were subsequently prepared for single cell RNA sequencing. Single cell suspensions were loaded onto 10X Genomics Single Cell 3' Chips along with the reverse transcription master mix as per the manufacturer's protocol for the Chromium Single Cell 3' v2 Library (10X Genomics; PN-120233), to generate single cell gel beads in emulsion. Libraries were then sequenced on an Illumina HiSeq 2500.

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Genetic signatures of retinal ganglion cells revealed through single cell profiling [dissociated RGC]

Project description:Genetic signatures of retinal ganglion cells revealed through single cell profiling [dissociated RGC]

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