Project description:RNA analysis of CRISPR/Cas9 ZNF519 knockout (KO), ZNF441 knockout (KO), ZNF468 knockout (KO) and wild type hESC-derived cortical organoids and ChIP-seq analysis of CRISPR/Cas9 ZNF519 knockout (KO) and wild type hESC-derived cortical organoids, and HEK293 cells with ZNF519 overexpression (OE).
Project description:We used Crispr/Cas9 technology to establish a homozygous clone of EphA2-SE deletion in tumor cells. Wild-type cells (WT) and homozygous cloned cells (EphA2-SE-/-) were selected for high-throughput data detection.
Project description:The goal of this study is to assess the role of ASH1 like histone lysine methyltransferase (ASH1L) in the biology of anaplastic thyroid cancer. CRISPR-Cas9 was used to create 4 independent cell lines derived from BHT-101 anaplastic thyroid cancer cells with premature stop codons prior to the catalytic domain within both alleles of ASH1L. RNA-seq was performed on these 4 KO cell lines, and compared to 3 biological replicates of wild type BHT-101 cells.
Project description:Actr3 WT (control) and Actr3 KO mIMCD3 cells were generated using CRISPR/Cas9 and independent gRNAs for control and KO cell lines. Single cells clones (Ctrl.-1, Crtl.-2, KO-1, KO-2) were selected and validated by western blotting confirming loss of Actr3 in KO cell lines on protein level. Transcriptome profiling (RNA-Sequencing) and differential gene expression analysis of independent replicates per cell line was performed.
Project description:Purpose: We idenficied a circRNA, circBNC2, that was downregulated during tubular epithelial cell (TEC) injury. To study the function of circBNC2, we performed CRISPR-Cas9 to knock out circBNC2 in HK2 (a well recognized TEC) cells. Next-generation Sequencing was used to identified the differentially expressed mRNA between circBNC2-KO HK2 cells and wild-type HK2 cells.
Project description:IDH3a KO in NHAs was demonstrated to exert global DNA methylation changes. These studies were followed by an Illumina EPIC methylation array to evaluate the location of differentially methylated CpGs
Project description:We generated a SNORD71 KO chondrocyte cell pool using CRISPR/Cas9 gene editing. A CRISPR control cell line was generated and used as a control. Levels of 2’-O-methylation of human rRNAs in SNORD71 KO cell pool and CRISPR control cells were evaluated by RiboMethSeq.
Project description:Purpose: We idenficied a circRNA, circBNC2, that was downregulated during hepatocytes injury. To study the function of circBNC2, we performed CRISPR-Cas9 to knock out circBNC2 in L-02 (a well recognized human hepatocytes) cells. Next-generation Sequencing was used to identified the differentially expressed mRNA between circBNC2-KO L-02 cells and wild-type L-02 cells.
Project description:Haploid human embryonic stem cells harboring TP53 or MLH1 knockout (KO) were subjected to a genome-wide CRISPR-Cas9 knockout screen to identify synthetic lethal interactions associated with the mentioned genes.
Project description:This study investigates the transcriptional consequences of Laminin-α5 (LAMA5) loss in human urine-derived stem cells (USCs) to understand the molecular etiology of idiopathic short stature. The experiment consists of two components: 1. Differentiation Profiling: CRISPR/Cas9-mediated LAMA5 knockout (KO) USCs and wild-type (WT) controls were profiled in three conditions: undifferentiated state, osteogenic differentiation, and chondrogenic differentiation. This analysis aims to interpret how cell-matrix interactions regulate lineage-specific transcriptional programs and spheroid architecture. 2. Pharmacological Rescue: To assess the functional role of canonical WNT signaling in LAMA5-mediated regulation, LAMA5 knockout USCs were undergoing chondrogenic differentiation were treated with the WNT agonist Lithium Chloride (LiCl) or left untreated. Data analysis identifies a LAMA5-dependent gene regulatory network enriched for limb morphogenesis factors (including WNT7A, PITX1, and FLI1) and demonstrates partial restoration of this network upon β-catenin stabilization via LiCl. Study Design / Samples Total Samples: 24 Organism: Homo sapiens Cell Type: Urine-derived stem cells (USCs) Detailed Sample List: Condition 1 (Genotype x Lineage): Comparison of WT vs. LAMA5 KO across three differentiation stages (n=18). 3x Wild-type (WT) - Undifferentiated 3x Wild-type (WT) - Osteogenic 3x Wild-type (WT) - Chondrogenic 3x LAMA5 KO - Undifferentiated 3x LAMA5 KO - Osteogenic 3x LAMA5 KO - Chondrogenic Condition 2 (Rescue Treatment): Effect of LiCl treatment on LAMA5 KO cells (n=6). 3x LAMA5 KO (Clone 2) - Untreated (Control) 3x LAMA5 KO (Clone 2) - Treated with LiCl
