Dataset Information


C. elegans PRG-1 co-IP small RNAs, 5p-end-independent

ABSTRACT: These datasets examine the association of small RNAs with 3p hydroxyls (including endo-siRNAs, miRNAs, and 21U-RNAs) with the PRG-1 protein through co-immunoprecipitation. Two datasets are provided; one sequences small RNAs that co-IP with PRG-1; the other sequences small RNAs from the IP input sample. These RNA samples were prepared using an approach that captures small RNAs independent of the covalent status of their 5p termini, as described in Ambros et al. (2003) Curr Biol 13:807-18. These datasets were prepared in the Craig C. Mello laboratory. Keywords: identification of PRG-1-associated small RNAs Overall design: CE-PRG1-coIP-Illumina 1 flowcell each for input, PRG-1 co-IP

INSTRUMENT(S): MIT Caenorhabditis elegans small RNA sequences (Illumina/Solexa)

SUBMITTER: James Graham Ruby 

PROVIDER: GSE11737 | GEO | 2008-06-16



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In metazoans, Piwi-related Argonaute proteins have been linked to germline maintenance, and to a class of germline-enriched small RNAs termed piRNAs. Here we show that an abundant class of 21 nucleotide small RNAs (21U-RNAs) are expressed in the C. elegans germline, interact with the C. elegans Piwi family member PRG-1, and depend on PRG-1 activity for their accumulation. The PRG-1 protein is expressed throughout development and localizes to nuage-like structures called P granules. Although 21U-  ...[more]

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