Genomics

Dataset Information

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Perturbation of the OGT-TET1 interaction in mouse embryonic stem cells (WGBS)


ABSTRACT: Whole genome bisulfite sequencing of wild type embryonic stem cells and cells bearing a mutation that perturbs the OGT-TET1 interaction Overall design: Whole genome bisulfite sequencing in wild type and Tet1 D2018A mutant mESCs

INSTRUMENT(S): Illumina HiSeq 4000 (Mus musculus)

SUBMITTER: Joel Hrit  

PROVIDER: GSE119665 | GEO | 2018-10-29

REPOSITORIES: GEO

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Publications


TET enzymes convert 5-methylcytosine to 5-hydroxymethylcytosine and higher oxidized derivatives. TETs stably associate with and are post-translationally modified by the nutrient-sensing enzyme OGT, suggesting a connection between metabolism and the epigenome. Here, we show for the first time that modification by OGT enhances TET1 activity in vitro. We identify a TET1 domain that is necessary and sufficient for binding to OGT and report a point mutation that disrupts the TET1-OGT interaction. We  ...[more]

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