Project description:We report the presence of extensive, transcriptionally controlled oscillations in the C. elegans, developmental transcriptome. Furthermore, using ribosome profiling, we show that these oscillating transcripts are actively translated. Examination of two timecourses that were collected over C. elegans development and analyzed by RNA-seq of "RiboMinus" libraries
Project description:We report the presence of extensive, transcriptionally controlled oscillations in the C. elegans, developmental transcriptome. Furthermore, using ribosome profiling, we show that these oscillating transcripts are actively translated.
Project description:We report cell type specific Nova HITS-CLIP using BAC-transgenic lines expressing GFP-Nova under the motor neuron specific choline acetyltransferase (Chat) promoter. By comparing transcriptome wide Nova binding map in motor neurons and that in the whole spinal cord, we identified differential Nova binding sites in motor neurons, which correlate with motor neuron specific RNA processing.
Project description:We detected 32230 transfrags by Tiling array Analysis Software, of which 5866 were transfrags with unknown function (TUF). These TUFs exhibited distinct chromosome location, expression and conservation patterns. Six developmental and two conditional stages of C. elegans 70~500nt transcriptome. L1~L4, mature adult (MA), male (ML), dauer (DU), heat shocked (HS). GSM591423_CB2007199-L4-1.CEL is Ce25b_MF, GPL5634 is Ce25b_MR, CEL file is okay using GPL5634's cdf.
Project description:We report the transcriptome profile of one sequenced sample of mRNA isolated from pooled (20 from each genotype) abdomen fly extracts enriched in fat body content of fat body-specific Sdc RNAi knockdown and control flies Abdominal fat body mRNA profiles of 4-6-day old control and fat body-specific Sdc RNAi knockdown were generated by deep sequencing using Illumina HiSeq 2500
Project description:We report genes induced under transient hypoxia in an ELT-2 dependent manner Adult C. elegans were exposed to transient hypoxia and fed ELT-2 RNAi. Normoxia and Empty Vector RNAi were performed as controls
