Project description:In order to understand age-associated changes in the transcriptome, we quantified the average gene expression profile of C. elegans nematodes at days 1-12 of life. Because of the high temporal resolution at which data was collected, these data can be used as a ground truth measurement for the transcriptomic determination of physiological age.
Project description:We report the gene expression in L1 arrest (48 hours) of wild-type sleeping worms (N2) and RIS-manipulated worms (HBR2340 and PHX2193)
Project description:We detected 32230 transfrags by Tiling array Analysis Software, of which 5866 were transfrags with unknown function (TUF). These TUFs exhibited distinct chromosome location, expression and conservation patterns. Six developmental and two conditional stages of C. elegans 70~500nt transcriptome. L1~L4, mature adult (MA), male (ML), dauer (DU), heat shocked (HS). GSM591423_CB2007199-L4-1.CEL is Ce25b_MF, GPL5634 is Ce25b_MR, CEL file is okay using GPL5634's cdf.
Project description:The presence of brain/spinal white matter lesions typical for multiple sclerosis (MS) in asymptomatic individuals is known as ‘radiologically isolated syndrome’ (RIS). Allow for the established fact that RIS patients are at high risk of MS development, understanding the causes underlying RIS onset and the variability of its conversion to MS are of great importance. In order to investigate RIS-specific transcription signature we perfromed high-throughput RNA sequencing in peripheral blood mononuclear cells (PBMCs) of 8 RIS patients and 8 age- and sex-matched healthy controls. We identified 57 differentially expressed genes (DEGs), which levels differed by more than 2 times when compared these groups (FDR p value < 0.05). Gene ontology enrichment analysis in the "biological process" category revealed 16 signalling pathways significantly overrepresented by identified DEGs. The most significant changes in gene expression in PBMCs of RIS patients occur in pathways involved in regulation of the immune response, cytokine and chemokine signaling, cytokine production, and leukocyte migration. In general, analyzing the global transcriptome we demonstrated the dysregulation of immune processes in PBMCs of RIS patients, confirming the current assumption that RIS represents the preclinical stage and/or subclinical form of MS.
Project description:We sequenced amplified mRNA from 20 pooled AB and 20 pooled P1 blastomeres hand isolated from 2-cell stage C. elegans embryos three replicates each. Transcriptome profiles of each blastomere at the 2-cell stage of the C. elegans early embryo.
