Reversible Histone Glycation Drives Disease-Associated Changes in Chromatin Architecture

ABSTRACT: Glycation is a covalent protein modification that accumulates in the cellular environment. Histone proteins are particularly susceptible to glycation due to their extremely long half-lives and nucleophilic disordered tails. Here, we performed ATAC-seq on 293T cells cultured in the presence or absence of 0.5mM methylglioxal, a reactive glycolytic intermediate, for 12 hours. We demonstrate that methylglioxal treatment is associated with changes in histone occupancy and chromatin architecture globally.

ORGANISM(S): Homo sapiens

PROVIDER: GSE121252 | GEO | 2019/02/14

REPOSITORIES: GEO