Gene expression profile in mouse skeletal muscle after Mettl21e deletion
Ontology highlight
ABSTRACT: Analysis of tibialis anterior (TA) muscle isolated from wildtype (WT) and Mettl21e deletion mice (KO). Results provide unbiased gene expression profile of TA muscle after Mettl21e deletion.
Project description:Muscle satellite cell (MuSCs)-specific Sirt7 knockout (KO) mice display impaired muscle regeneration after injury. To evaluate the effect of SIRT7 deficiency on the early stages of muscle regeneration in vivo, we performed RNA-seq analysis for tibialis anterior (TA) muscle of control and MuSCs-specific Sirt7 KO mice at the early stages of myogenesis, day 3 post injury.
Project description:samples from mouse Skeletal Muscle, analysis different expression profiling<br>HDAC4:Sciatic nerve transection model was prepared after injection of HDAC4-shRNA lentivirus into the tibialis anterior muscle of mice. 14 days later, tibialis anterior muscle was obtained. DenSciatic nerve transection model was prepared after injection of empty vector virus into the tibialis anterior muscle of mice. 14 days later, tibialis anterior muscle was obtained. N: The empty vector virus was injected into muscles from the sham group. 14 days later, tibialis anterior muscle was obtained.
Project description:mRNA expression data for the mouse tibialis anterior (TA) muscle (n=5 per group) were obtained using Affymetrix Mouse Clariom S Assay (Affymetrix, Santa Carla, CA) according to the manufacturer’s recommendations.
Project description:Gadd45a is a stress-induced protein that causes skeletal muscle atrophy. The goal of these studies was to determine the effects of Gadd45a overexpression on mRNA levels in mouse skeletal muscle. For additional details see Ebert et al, Stress-Induced Skeletal Muscle Gadd45a Expression Reprograms Myonuclei and Causes Muscle Atrophy. JBC epub. June 12, 2012. Tibialis anterior (TA) muscles from muscle-specfic ATF4 knockout mice (ATF4 mKO) were transfected with either 20 mg empty plasmid (pcDNA3) (left TA) or 20 mg pCMV-FLAG-Gadd45a (right TA) and harvested 7 days later. mRNA levels in Gadd45a-transfected muscles were normalized to levels in control transfected muscles.
Project description:Analysis of soleus (SOL) muscle isolated from wildtype (WT) and Mettl21cLacZ/LacZ mice (KO). Results provide unbiased gene expression profile of SOL muscle after Mettl21c deletion.
Project description:PGC1b transgenic mice were generated to selectively over-express PGC1b in skeletal muscles using human skeletal alpha-actin gene promoter. The gene expression profiles were collected from Tibialis anterior (TA) muscles of wild type (WT) and PGC1b transgenic (TG) mice. Tibialis anterior muscles from three month old WT and PGC1b transgenic male mice.
Project description:Desmin is a cytoskeletal protein in muscle involved in integrating cellular space and transmitting forces. In this study we sought to determine the effects of desmin deletion on skeletal muscle at the transcriptional level across many pathways of muscle physiology. RNA was isolated from the TA muscle of mice of two genotypes (wildtype (WT) and desmin knockout (KO)) and two ages (7-9 weeks (Adult) and 12-14 months (Aged)). Numbers per group are as follows: WT_Adult (5), WT_Aged (5), KO_Adult (5), KO_Aged (4).
Project description:To investigate the role of the circadian clock gene Bmal1 in skeletal muscle, we compared the circadian transcriptomes of fast tibialis anterior (TA) and slow soleus (SOL) skeletal muscles from muscle-specific Bmal1 KO (mKO) and their control Cre- littermates (Ctrl). Keyword: Circadian Transcriptome, time course