Genomics

Dataset Information

42

Cap-specific terminal N6-methylation of RNA mediated by RNA polymerase II-associated methyltransferase


ABSTRACT: N6-methyladenosine (m6A) is a major post-transcriptional modification in mRNAs, and plays critical roles in RNA metabolism and function. In addition to the internal m6A, N6, 2’-O-dimethyladenosine (m6Am) is present at the transcription start nucleotide of capped mRNAs in vertebrates. Biogenesis and functional role of m6Am remain to be investigated. Using a reverse genetic approach, we here identified PCIF1, a factor that interacts with phosphorylated C-terminal domain of RNA polymerase II, to be a SAM-dependent methyltransferase responsible for N6-methylation of m6Am. Thus, we named it as cap-specific adenosine methyltransferase (CSAM). Structural study of CSAM complexed with substrates revealed a molecular basis of cap-specific m6A formation mediated by a novel helical domain. Transcriptome-wide analysis revealed that N6-methylation of m6Am has a functional role in up-regulating translational activity of capped mRNAs. CSAM knockout cell exhibited an apparent sensitivity to oxidative stress. Overall design: Ribosome profiling and RNA-seq

INSTRUMENT(S): Illumina HiSeq 4000 (Homo sapiens)

SUBMITTER: Shintaro Iwasaki  

PROVIDER: GSE122071 | GEO | 2018-11-02

REPOSITORIES: GEO

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