Genomics

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M6A/m-Seq of mouse adult cortex of Mettl3 cKO or Fto cKO mice


ABSTRACT: N6-Methyladenosine (m6A) and N6,2′-O-dimethyladenosine (m6Am) are abundant mRNA modifications that regulate transcript processing and translation. The role of both, here termed m6A/m, in the stress response in the adult brain in vivo are currently unknown. Here, we investigated the effect of gene deletion of Mettl3, a m6A methyltransferase, and Fto, a m6A and m6Am demethlyase, induced in adulthood in excitatory neurons of the neocortex and hippocampus (Camk2a-Cre Mettl3 or Fto cKO) on the cortical epitranscriptome. PolyA-RNA-fragments from 3-5 replicates per group were processed both as m6A/m-sample (RNA immunoprecipiation RIP with an m6A and m6Am antibody) and RNA-input sample. Overall design: 5 replicates of WT mice (from both lines) as well as 3 replicates of Mettl3 cKO and 3 replicates of Fto cKO mice (cortex sample from 3 mice pooled per replicate) were processed as m6A/m-immunoprecipitation-sample ("m6A") or as input RNA samples ("RNA"). Additionally, 1 RNA sample (equimolar mixed from all samples) was processed as IgG control.

INSTRUMENT(S): Illumina HiSeq 4000 (Mus musculus)

SUBMITTER: Mareen Engel  

PROVIDER: GSE113793 | GEO | 2018-07-10

REPOSITORIES: GEO

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