Genomics

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M6A/m-Seq of mouse adult cortex after acute stress


ABSTRACT: N6-Methyladenosine (m6A) and N6,2′-O-dimethyladenosine (m6Am) are abundant mRNA modifications that regulate transcript processing and translation. The role of both, here termed m6A/m, in the stress response in the adult brain in vivo are currently unknown. Here, we provide a detailed analysis of the stress-epitranscriptome using m6A/m-Seq of 10-12 w male C57BL/6 mouse cortex of unstressed or acutely stressed mice (4 h after 15 min restraint stress) of 6-7 samples per condition (pooled from 3 mice each). PolyA-RNA-fragments of each sample was processed both as m6A/m-sample (RNA immunoprecipiation RIP with an m6A and m6Am antibody) and RNA-input sample. Overall design: 7 replicates for basal mouse cortex ("control", "C"), 6 replicates for stressed mouse cortex (4 h after 15 min of restraint stress, "stress", "S"), each samples was processed as m6A/m-immunoprecipitation-sample ("m6A") or as input RNA samples ("RNA"). Additionally, 1 RNA sample (equimolar mixed from all samples) was processed as IgG control.

INSTRUMENT(S): Illumina HiSeq 4000 (Mus musculus)

SUBMITTER: Mareen Engel  

PROVIDER: GSE113781 | GEO | 2018-07-10

REPOSITORIES: GEO

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