Dataset Information


M6A/m-Seq of mouse adult cortex after acute stress

ABSTRACT: N6-Methyladenosine (m6A) and N6,2′-O-dimethyladenosine (m6Am) are abundant mRNA modifications that regulate transcript processing and translation. The role of both, here termed m6A/m, in the stress response in the adult brain in vivo are currently unknown. Here, we provide a detailed analysis of the stress-epitranscriptome using m6A/m-Seq of 10-12 w male C57BL/6 mouse cortex of unstressed or acutely stressed mice (4 h after 15 min restraint stress) of 6-7 samples per condition (pooled from 3 mice each). PolyA-RNA-fragments of each sample was processed both as m6A/m-sample (RNA immunoprecipiation RIP with an m6A and m6Am antibody) and RNA-input sample. Overall design: 7 replicates for basal mouse cortex ("control", "C"), 6 replicates for stressed mouse cortex (4 h after 15 min of restraint stress, "stress", "S"), each samples was processed as m6A/m-immunoprecipitation-sample ("m6A") or as input RNA samples ("RNA"). Additionally, 1 RNA sample (equimolar mixed from all samples) was processed as IgG control.

INSTRUMENT(S): Illumina HiSeq 4000 (Mus musculus)

SUBMITTER: Mareen Engel  

PROVIDER: GSE113781 | GEO | 2018-07-10


Dataset's files

Action DRS
GSE113781_processed_data_files.tar.gz Other
Items per page:
1 - 1 of 1

Similar Datasets

2018-07-10 | GSE113789 | GEO
2018-07-10 | GSE113793 | GEO
2018-01-01 | S-EPMC6151148 | BioStudies
2015-06-29 | E-GEOD-63753 | BioStudies
2019-01-01 | S-EPMC6386707 | BioStudies
2019-01-01 | S-EPMC6904561 | BioStudies
2019-01-01 | S-EPMC6688901 | BioStudies
2015-01-01 | S-EPMC4487409 | BioStudies
2019-01-01 | S-EPMC6703822 | BioStudies
2018-07-10 | GSE113798 | GEO