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Rheumatoid arthritis associated microRNA in peripheral blood mononuclear cells

ABSTRACT: Genome wide microRNA profiling in peripheral blood mononuclear cells from rheumatoid arthritis (RA) patients and healthy controls. The Affymetrix miRNA 4.0 chip was used to obtain RNA profiles in 28 RA patients and 18 healthy controls. Overall design: To identify novel rheumatoid arthritis (RA) associated microRNAs and comprehensively understand their underlying pathologic mechanism, we compared the Affymetrix miRNA 4.0 microarray data from PBMCs of 28 RA patients with that of 18 healthy controls.

INSTRUMENT(S): [miRNA-4] Affymetrix Multispecies miRNA-4 Array [ProbeSet ID version]

ORGANISM(S): Homo sapiens

SUBMITTER: Xingbo Mo

PROVIDER: GSE124373 | GEO | 2020-03-17

REPOSITORIES: GEO

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Publications

Epigenetically regulated co-expression network of genes significant for rheumatoid arthritis.

He Pei P Mo Xing-Bo XB Lei Shu-Feng SF Deng Fei-Yan FY

Epigenomics 20191106 14

<b>Aim:</b> To identify epigenetically regulated network of genes in peripheral blood mononuclear cells significant for rheumatoid arthritis (RA). <b>Methods:</b> Differentially expressed genes (DEGs) and their associated differentially expressed miRNAs and differentially methylated positions (DMPs) were identified. Causal inference test (CIT) identified the causal regulation chains. The analyses, for example, weighted gene co-expression network (WGCNA), protein-protein interaction and functiona ...[more]

PMID: 31693422

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Rheumatoid arthritis associated DNA methylation sites in peripheral blood mononuclear cells

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Comparison of microRNA expression profiles of Kashin-Beck disease, osteoarthritis and rheumatoid arthritis.

Project description:Kashin-Beck disease (KBD) is a chronic osteochondropathy with unclear pathogeny. In this study, we compared the microRNA expression profiles of 16 KBD patients, 16 osteoarthritis (OA) patients and 16 rheumatoid arthritis (RA) patients and 16 healthy controls in their blood specimens. miRNAs expression profiling was performed using Exiqon miRCURY LNATM miRNAs Array. miRNAs target genes were predicted using miRror suite. Another independent mRNA expression profile dataset of 20 KBD patients and 15 healthy controls were integrated with the miRNA expression profiles of KBD. We identified 140 differently expressed miRNAs in KBD vs.GO enrichment analysis found that hypoxia, Wnt receptor signaling pathway and vitamin B6 biosynthesis related GO terms were significantly overrepresented in the target genes of differently expressed miRNAs in KBD vs.18 differently expressed common miRNAs were identified in KBD vs. Control, KBD vs. OA and KBD vs. RA. Integrating the lists of differently expressed miRNA target genes and mRNA differently expressed genes detected 6 common genes for KBD. Our results demonstrated the altered miRNAs expression profiles of KBD comparing to healthy controls, OA and RA, which provide novel clues for clarifying the mechanism of KBD.

2017-01-01 | S-EPMC5428653 | BioStudies

MicroRNA Expression Analysis of Centenarians and Rheumatoid Arthritis Patients Reveals a Common Expression Pattern.

Project description:Micro-RNA (miRNA) are a family of small non-coding ribonucleic acids that inhibits post-transcriptionally the expression of their target messenger RNA (mRNA). We are interested in studying the involvement of miRNA in longevity and autoimmune diseases. In this study we compared the different expression of seven microRNAs between human plasma healthy controls, plasma samples of centenarians and samples from patients with rheumatoid arthritis. We used the Life Technologies' protocol to quantify seven miRNAs from 62 plasma samples: 20 healthy human controls, 14 centenarians, 28 patients with rheumatoid arthritis. TaqMan MicroRNA assays were used to analyze the expression profiles of miR-125b-5p, miR-425-5p, miR-200b5p, miR-200c-3p, miR-579-3p, miR-212-3p, miR-21-5p and miR-126-3p. The relative expression of mature miRNAs was analyzed using software REST. Our results show that miR-425-5p, miR-21 and miR-212 significantly decreased in centenarians and in patients with rheumatoid arthritis compared with controls. Furthermore in this work we highlight a connection between corticosteroid treatment and miRNAs expression.

2017-01-01 | S-EPMC5562112 | BioStudies

miRNA expression profile associated with response to Tripterygium wilfordii Hook F in the treatment of rheumatoid arthritis

Project description:miRNA expression profile in peripheral blood mononuclear cells obtained from a discovery cohort The discovery cohort includes12 RA patients (6 responders and 6 non-responders) treated with TwHF was detected by Affymetrix miRNA 4.0 arrays. Overall design: To identify potential serum microRNA biomarkers, which are predictive of rheumatoid arthritis patients' response to TwHF.

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2020-01-01 | S-EPMC7153549 | BioStudies

circulating microRNA expression profiles of Kashin-Beck disease, osteoarthritis and rheumatoid arthritis

Project description:We compared the circulating microRNA expression profiles of KBD, osteoarthritis (OA), rheumatoid arthritis (RA) and healthy controls. Blood specimens were collected from 3 KBD patients, 3 OA patients, 3 RA patients and 3 healthy controls. miRNAs expression profiling was performed using Exiqon miRCURY LNATM miRNAs Array.

2016-06-01 | E-MTAB-3514 | ArrayExpress

Hormone, metabolic peptide, and nutrient levels in the earliest phases of rheumatoid arthritis-contribution of free fatty acids to an increased cardiovascular risk during very early disease.

Project description:Rheumatoid arthritis (RA) is a chronic autoimmune disease associated with changes in several hormones and metabolic peptides. Crosstalk between these factors and the immune system may be important for homeostasis during inflammation. Here, we studied the levels of hormones, metabolic peptides, and nutrients in individuals at risk for developing RA (at risk). In total, 18 hormones, metabolic peptides, and nutrients were measured in fasting serum samples from 45 autoantibody-positive individuals at risk, 22 RA patients, and 16 healthy subjects. Triglyceride (TG) levels were also measured in an independent validation cohort of 32 individuals at risk, 20 early arthritis patients, and 20 healthy controls. We found an elevated TG level in individuals at risk and significantly higher TG levels in RA patients compared to healthy controls. These results were confirmed in the validation cohort. Similarly, free fatty acid (FFA) levels showed an increase in individuals at risk and were significantly higher in RA patients compared to healthy controls. In RA patients, FFA levels were positively correlated with disease activity. Pancreatic polypeptide (PP) and norepinephrine levels were highly significantly increased in individuals at risk and RA patients compared to healthy controls. TG and FFA levels are increased in RA patients and positively correlated with disease activity parameters. The results presented here suggest a role for FFAs in the pathogenesis of RA. Furthermore, PP and norepinephrine may be a biomarker that could assist in the identification of individuals at risk.

2017-01-01 | S-EPMC5290053 | BioStudies

Development of a Novel Diagnostic Biomarker Set for Rheumatoid Arthritis Using a Proteomics Approach.

Project description:Background:Rheumatoid arthritis (RA) is an autoimmune disease that starts with inflammation of the synovial membrane. Studies have been conducted to develop methods for efficient diagnosis of RA and to identify the mechanisms underlying RA development. Blood samples can be useful for detecting disturbance of homeostasis in patients with RA. Nanoliquid chromatography-tandem mass spectrometry (LC-MS/MS) is an efficient proteomics approach to analyze blood sample and quantify serum proteins. Methods:Serum samples of 18 healthy controls and 18 patients with RA were analyzed by LC-MS/MS. Selected candidate biomarkers were validated by enzyme-linked immunosorbent assay (ELISA) using sera from 43 healthy controls and 44 patients with RA. Results:Thirty-eight proteins were significantly differentially expressed by more than 2-fold in healthy controls and patients with RA. Based on a literature survey, we selected six candidate RA biomarkers. ELISA was used to evaluate whether these proteins effectively allow distinguishing patients with RA from healthy controls and monitoring drug efficacy. SAA4, gelsolin, and vitamin D-binding protein were validated as potential biomarkers of RA for screening and drug efficacy monitoring of RA. Conclusions:We identified a panel of three biomarkers for RA which has potential for application in RA diagnosis and drug efficacy monitoring. Further, our findings will aid in understanding the pathogenesis of RA.

2018-01-01 | S-EPMC6312602 | BioStudies

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2016-06-01 | E-MTAB-3514 | BioStudies

The pathogenic role of stem cell-like memory T cells in rheumatoid arthritis

Project description:Objective: Stem cell-like memory T cells (Tscm) are a subset of memory T cells that have the characteristics of stem cells. The role of Tscm cells in rheumatoid arthritis (RA) is unknown. Methods: The transcriptomes of Tscm cells from RA patients were compared with those from HCs. Results: Transcriptome analysis revealed that Tscm cells from RA patients showed patterns distinct from those of HCs. Conclusion: The percentage of transcriptionally distinct and potentially pathogenic Tscm cells are higher in RA patients than in HCs; these cells may be a continuous source of pathogenic T cells, which perpetuate RA. Overall design: mRNA profiles of Tscm cells sorted from PBMCs of healthy controls or severe rheumatoid arthritis patients or rheumatoid arthritis in remission were generated by RNA sequencing.

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