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Mesenchymal (MES)- and Adrenergic (ADN) neuroblastoma cell line pair of isogenic origin

ABSTRACT: Cell line pair with different phenotypes were isolated from 1 patient to study intra-tumor heterogeneity in neuroblastoma

ORGANISM(S): Homo sapiens

PROVIDER: GSE125057 | GEO | 2021/10/01

REPOSITORIES: GEO

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Mesenchymal (MES)- and Adrenergic (ADRN) neuroblastoma cell line pairs of isogenic origin

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Mesenchymal (MES)- and Adrenergic (ADN) neuroblastoma cell line pairs of isogenic origin

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Project description:Gene mutations in components of SWI/SNF chromatin-remodeling complexes are found in approximately 20% of all human cancers, with ARID1A being the most frequently mutated subunit. In neuroblastoma, sequence alterations of ARID1A were identified in 6% of primary tumors and cell lines, and this gene is hemizygously deleted in at least 87% of cases with chromosome 1p deletion, which almost always accompanies MYCN gene amplification and is the most common deletions in high-risk subtypes of this tumor. However, the role of ARID1A haploinsufficiency in neuroblastoma pathogenesis and the mechanisms involved remain unclear. Here, we show that disruption of ARID1A homologs in a zebrafish model accelerates the onset and increases the penetrance of MYCN-driven neuroblastoma by increasing cell proliferation in the sympathoadrenal lineage. Depletion of ARID1A in human NGP neuroblastoma cells promoted the adrenergic-to-mesenchymal transition with changes in enhancer-mediated gene expression due to alterations in the genomic occupancies of two distinct SWI/SNF assemblies, BAF and PBAF. Our findings indicate that ARID1A is a haploinsufficient tumor suppressor in MYCN-driven neuroblastoma, whose depletion enhances tumor development and promotes the emergence of the more drug resistant mesenchymal cell state.

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