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Transcriptome sequencing to unravel the molecular mechanisms underlying the cuticle liquefaction of Antheraea pernyi following Antheraea pernyi nucleopolyhedrovirus challenge

ABSTRACT: Purpose: The goal of this study is to analyze the transcriptome profile changes of the cuticles between ApNPV-infected and non-infected A. pernyi larvae for investigating themolecular mechanisms of cuticle liquefaction of A. pernyi induced by ApNPV infection. Methods:The A. pernyi cuticles infected by ApNPV and normal samples were generated by deep sequencing, in triplicate, using Illumina Hiseq 4000. The high-quality reads were obtained by removing the reads that contained adaptor contamination, low quality bases and undetermined bases.The transcriptome were de novo assembly. Results:The RNA-seq data generated 57332226, 48859400, 50197630 and 52086538, 52447736, 47585326 raw reads from ApNPV-infected and control groups, respectively. After data filtering, 55715544, 48130252, 49354556, 51102012, 51633978, 46798094 clean reads were obtained (Table1). Using the Trinity de novo assembly programme, all short-read sequences were assembled into 87036 transcripts and 50126 unigenes. Conclusions:We systematically analyzed the gene expressional profiles and obtained both up- and down-regulated genes in ApNPV-infected cuticle compared with control. Meanwhile, we screened the chitin metabolism-related differentially expressed genes (DEGs) from the transcriptome data, and further characterized those DEGs based on their expression profiles challenged by ApNPV. Our research findings lay the foundation for further research on the molecular mechanisms of liquefaction induced by viral infection in insect.

ORGANISM(S): Antheraea pernyi

PROVIDER: GSE125821 | GEO | 2019/01/30

REPOSITORIES: GEO

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Unravelling the cellular and humoral immune responses of Antheraea pernyi hemolymph to Antheraea pernyi nucleopolyhedrovirus infection

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2019-01-29 | GSE125799 | GEO

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2019-01-29 | GSE125798 | GEO

Identification of early response genes to Pratylenchus coffeae in resistant ramie by digital gene expression analysis (leaves)

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2009-11-05 | GSE18878 | GEO

Expression profile of cuticular genes of silkworm, Bombyx mori

2009-11-04 | E-GEOD-18878 | biostudies-arrayexpress

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Project description:Comparative transcriptome analysis was performed to study differentially expressed genes (DEGs) between Cocytodes caerulea Guenée challenged (CH) and unchallenged (CK) leaves of ramie.We obtained 40.2 and 62.8 million reads for CH and CK libraries respectively.De novo assembling of these reads generated 26,759 and 29,988 unigenes respectively. After a integrate assembly for all data of these two libraries, a total of 46,533 unigenes with an average length of 845 bp were obtained.A total of 1179 genes were identified as DEGs, 657 and 1230 of them were up- and down- regulated respectively, in response to C.caerulea infestation.

2015-03-04 | GSE66447 | GEO

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2014-07-29 | E-GEOD-57326 | biostudies-arrayexpress

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