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PCMV-FAK vs pCMV in HU

ABSTRACT: CMV-driven FAK overexpression in soleus muscle was compared vs empty transfection in contralateral muscle after unloading

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE12745 | GEO | 2009/12/31

SECONDARY ACCESSION(S): PRJNA123159

REPOSITORIES: GEO

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pCMV-FAK vs pCMV in HU

Project description:CMV-driven FAK overexpression in soleus muscle was compared vs empty transfection in contralateral muscle after unloading paired comparison in contralateral leg

2010-06-24 | E-GEOD-12745 | biostudies-arrayexpress

pCMV-FAK vs pCMV in CTL

Project description:CMV-driven FAK overexpression in soleus muscle was compared vs empty transfection in contralateral muscle

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pCMV-FRNK/pCMV-FAK vs pCMV-FAK in CTL

Project description:CMV-driven inhibition of FAK in soleus muscle was compared vs contralateral muscle

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Integrated Proteomic and Metabolomic Analysis of Muscle Atrophy Induced by Hindlimb Unloading

Project description:Skeletal muscle atrophy, which is induced by factors such as disuse, spaceflight, certain medications, neurological disorders, and malnutrition, is a global health issue lacking effective treatment. Hindlimb unloading is a commonly used model for muscle atrophy. However, the underlying mechanism of muscle atrophy induced by hindlimb unloading remains unclear, particular from the perspective of myocyte proteome and metabolism. We first used mass spectrometry for proteomic sequencing and untargeted metabolomics to analyze soleus muscle changes in rats with hindlimb unloading. The study found 1052 proteins and 377 metabolites (with MS2 name) differentially expressed between HU group and CON group. Proteins like ACTN3, MYH4, MYBPC2, and MYOZ1, typically found in fast-twitch muscles, were upregulated, along with metabolism-related proteins GLUL, GSTM4, and NDUFS4. Metabolites arachidylcarnitine and 7,8-dihydrobiopterin, and pathways like histidine, taurine, and hypotaurine metabolism were linked to muscle atrophy. Protein and metabolism joint analysis revealed that some pathways such as glutathione metabolism, ferroptosis and lysosome pathways were likely to be involved in soleus atrophy. In this study, we have applied integrated deep proteomic and metabolomic analysis. The upregulation of proteins which are expressed in fast-twitch fibers indicated the conversion of slow-twitch fibers to fast-twitch fibers under HU. Some metabolism-related proteins have been screened out. Besides, some differentially abundant metabolites and pathways revealed the important role of metabolism in the muscle atrophy of soleus. Our study provides insights into the pathogenesis and treatment of muscle atrophy that results from unloading by integrating the proteomics and metabolomics of soleus muscles.

2025-05-07 | PXD057671 | Pride

RNA-Seq transcriptome profiling of soleus from adult and aged mice following disuse muscle atrophy and recovery

Project description:Rodent hind limb unloading was used as a model for reduced muscle activity and eventual atrophy. After a 10 day period of unloading, mice in this study were “reloaded” for 3 days and regained use of their hind limbs. We report the application of Next-generation sequencing (NGS) technology for high-throughput profiling of mRNA in soleus muscle of adult (6 mo) and aged (22-24 mo) mice. Our goal was to determine the effects of hind limb unloading and reloading on mRNA profiles in soleus muscle and compare between adult and aged mice. We find that there are distinct response in the profile of fatty acid oxidation, TCA cycle, ETC oxidative phosphorylation gene expression patterns in response to unloading and reloading. The repsonses are generally simialr between young and old mice.

2018-05-15 | GSE102284 | GEO

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