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Adenovirus vs Mock Infection

ABSTRACT: human bronchial smooth muscle cells were either infected with empty adenoviral type 5 vector at 40 MOI for 4 days, or Mock infected. Each sample set was then stimulated with a mixture of 10ng/mL IL-1beta, TNF-alfa, and gamma-IFN for 20 hours prior to RNA harvest. RNA samples were split, and the series consists of 3 slides, 2 of which are dye flipped. Keywords: parallel sample

ORGANISM(S): Homo sapiens

PROVIDER: GSE1291 | GEO | 2004/04/09

SECONDARY ACCESSION(S): PRJNA90207

REPOSITORIES: GEO

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Adenovirus vs Mock Infection

Project description:human bronchial smooth muscle cells were either infected with empty adenoviral type 5 vector at 40 MOI for 4 days, or Mock infected. Each sample set was then stimulated with a mixture of 10ng/mL IL-1beta, TNF-alfa, and gamma-IFN for 20 hours prior to RNA harvest. RNA samples were split, and the series consists of 3 slides, 2 of which are dye flipped.

2010-06-09 | E-GEOD-1291 | biostudies-arrayexpress

HSP27 3A vs WT

Project description:human bronchial smooth muscle cells were infected with adenoviral constructs containing either the phosphorylation mutant form of HSP27 (HSP27-3A) or the wild type form (HSP27-WT) at 40 MOI for 4 days. All sets were stimulated with a cytokine mixture containing 10 ng/ml IL-1beta, TNF-alfa, and gamma-IFN for 20 hours prior to harvest. This series represents 4 separate preparations of cells and RNA, with each set being subjected to a dye flip and hybridized to 2 different slides. Keywords: parallel sample

2004-04-09 | GSE1292 | GEO

HSP27 3A vs WT

2010-06-09 | E-GEOD-1292 | biostudies-arrayexpress

Cytokine Stimulated Vs Unstimulated

Project description:human bronchial smooth muscle cells were growth arrested. One group was stimulated with 10ng/mL of IL-1beta, TNF-alfa, and gamma IFN for 20 hours. For every cDNA sample, 2 dyeflipped slides were hybridized (these have the same date in the titles). All cy3 (channel 2) measurements were normalized to the dye coupling control before a ratio of the values was taken. Keywords: parallel sample

2004-04-09 | GSE1288 | GEO

Cytokine Stimulated Vs Unstimulated

2010-06-09 | E-GEOD-1288 | biostudies-arrayexpress

Transcription profiling of mouse fibroblast cells infected with adenoviral vector or mock infected

Project description:The innate immune response to 1st generation adenoviral vectors was ascertained through high level infection of primary cells from a inbred mouse strain, replete with an intact and functioning TLR system. Experiment Overall Design: Quiescent murine C57/Bl6 fibroblasts were Ad (E1-E3-LacZ at MOI=300) or Mock infected. Sixteen hrs post-infection, RNA was harvested, transcribe to cDNA and bitotin-labeled, and hybridized to Mu11k A and B arrays.

2007-12-04 | E-GEOD-3172 | biostudies-arrayexpress

Deep sequencing analysis of differences in miRNA expression between IBDV-infected and Mock-infected DF-1 cells

Project description:To evaluate the change of miRNA expression profile in DF-1 cells in respond to Infectious bursal disease virus (IBDV) infection, Deep sequencing was performed by LC Sciences (Hangzhou, China) on DF-1 cells infected with mock or IBDV Lx strain at an MOI of 1 for 24 h.

2017-10-13 | GSE90095 | GEO

Adenovirus infected murine embryonic fibroblasts

2005-10-20 | GSE3172 | GEO

Genes regulated by SOX2 overexpression in gastric cancer cells

Project description:To identify candidate downstream target genes of SOX2 in gastric cancer cells, we transiently expressed exogenous SOX2 in a gastric cancer cell line NUGC3 and analyzed significant changes of gene expression. NUGC3 cells were infected with the optimum 20 MOI (infectious units/cell) of SOX2- or control GFP-expressing adenoviral vectors. At 72 h after infection, the cells were harvested for cDNA microarray analysis.

2011-02-02 | E-GEOD-23589 | biostudies-arrayexpress

Response of mouse liver to Adenovirus, Helper Dependent Adenovirus, and Adeno-Associated Virus

Project description:Understanding host responses to viral gene therapy vectors is necessary for the development of safe and efficacious in vivo gene transfer agents. We describe the use of high-density spotted complementary DNA microarrays in monitoring the in vivo host transcriptional responses in mouse liver upon administration of either a "first-generation"adenoviral (Ad) vector, a helper-dependent "gutless" adenoviral (HD) vector, or an adeno-associated viral (AAV) vector containing human factor IX (hFIX) expression cassettes. Since HD and AAV do not contain any viral genes, they allow us to assess the host response to the viral capsid and packaged nonviral DNA in whole animals. Comparison of the host response to Ad and HD helps assess the importance of leaky adenoviral gene expression. While all three vectors induced characteristic temporally sequenced programs of gene expression, the gene expression programs induced by the Ad and HD adenovirus vectors were remarkably similar, including the induction of a prominent type I interferon (IFN)-dependent cluster within 6 hours of administration. In contrast, the AAV-based vector caused far fewer alterations of host-gene expression. Our results indicate that recognition of the Ad capsid or double-stranded DNA (of nonviral origin) in the vector elicits a robust type I IFN response that is, however, not elicited by AAV-derived vector transduction.

2009-05-13 | GSE16047 | GEO

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