Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Profiling of Naïve (CD45RA+CD28+), EM (CD45RA-CD28-) and TEMRA (CD45RA+CD28-) CD8+ T cells

ABSTRACT: RNAseq of 3 CD8 subsets (NAIVE, TEMRA and EM) from 8 paired Healthy Volunteers

ORGANISM(S): Homo sapiens

PROVIDER: GSE129356 | GEO | 2020/05/06

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Profiling of Naïve (CD45RA+CD28+), EM (CD45RA-CD28-) and TEMRA (CD45RA+CD28-) CD8+ T cells from kidney transplanted patients

Project description:RNAseq of 3 CD8 subsets (NAIVE, TEMRA and EM) from kidney transplanted patients

2022-08-24 | GSE198296 | GEO

Profiling of Naïve (CD45RA+CD28+), EM (CD45RA-CD28-) and TEMRA (CD45RA+CD28-) CD8+ T cells

Project description:Profiling of Naïve (CD45RA+CD28+), EM (CD45RA-CD28-) and TEMRA (CD45RA+CD28-) CD8+ T cells

| PRJNA530997 | ENA

Transcription profiling of human CD8 naive (TN), central memory (TCM), effector memory (TEM), and effector memory RA (TEMRA) T cells

Project description:Memory T cells are heterogeneous in terms of their phenotype and functional properties. We investigated the molecular profiles of human CD8 naïve (TN), central memory (TCM), effector memory (TEM), and effector memory RA (TEMRA) T cells using gene expression microarrays and phospho-protein-specific intracellular flow cytometry. We demonstrate that TCM have a gene expression and cytokine signaling signature that lies between that of TN and TEM or TEMRA cells, whereas TEM and TEMRA are closely related. Our data define the molecular basis for the different functional properties of central and effector memory subsets. We show that TEM and TEMRA cells strongly express genes with known importance in CD8 T cell effector function. In contrast, TCM are characterized by high basal and cytokine-induced STAT5 phosphorylation, reflecting their capacity for self-renewal. Altogether, our results distinguish TCM and TEM/TEMRA at the molecular level and are consistent with the concept that TCM represent memory stem cells.

2005-01-01 | E-TABM-40 | biostudies-arrayexpress

Genome-wide maps of chromatin state in CD8 Naïve T cells upon differentiation and subsequent reversion to Naïve-like phenotype

Project description:We have employed whole genome ATAC-seq to profile the chromatin accssibility landscape of naive CD8+ T cells (TN) and these same cells following activation and differentiation to CM/EM and following incubation with homeostatic cytokines, a reverted naive-like phenotype (TNrev). Four umbilical cord blood samples were used to enrich for TN and to produce the respective EM CM and TNrev phenotypes.

2020-04-30 | GSE120618 | GEO

Gene expression profiles of human CD45RA+CCR7-CD8+ T cells

Project description:Human CD8+ T cells are functionally heterogeneous and can be divided into distinct subsets according to CCR7 and CD45RA expression levels. Among the subsets, CCR7-CD45RA+ CD8+ T cells are considered to be terminally differentiated cells and designated as Temra. Temra show attenuated ability to proliferate and produce IFN-gamma in response to TCR stimulation, while Temra show improved function after IL-15 treatment. To clarify the transcriptional signatures induced by the stimulations, Temra were purified using flow cytometry, stimulated with IL-15 or with anti-CD3/CD28 (TCR stimulation), or cultured without stimulation for 2 days, and subjected to microarray analysis.

2015-12-01 | GSE69072 | GEO

RNA-seq of KIR3DL3+ and KIR3DL3- CD8 terminally differentiated effect memory T cells (TEMRA)

Project description:The majority (60-97%) of KIR3DL3+ CD8 T cells belongs to TEMRA. To understand the function and characteristic of KIR3DL3+ CD8 T cells, we performed RNA-seq of KIR3DL3+ and KIR3DL3- CD8 TEMRA cells

2021-05-27 | GSE175550 | GEO

Epigenetic regulation of the transcriptional program in memory and terminally differentiated CD8+ T cells [HCAFIS_07_Gene_Expression]

Project description:Epigenetic mechanisms play a critical role during differentiation of T cells by contributing to the formation of stable and heritable transcriptional patterns. To further study the mechanisms of memory maintenance in CD8+ T cells, we performed genome-wide analysis of DNA methylation, histone marking (H3K9Ac and H3K9me3) and gene expression profiles in naive, effector memory (EM) and terminally differentiated memory (TEMRA) cells. Our results indicate that DNA demethylation and histone acetylation are coordinated to generate the transcriptional program associated with memory cells. Conversely, EM and TEMRA cells share a very similar epigenetic landscape. Nonetheless, the TEMRA transcriptional program predicts an innate immunity phenotype associated with genes never reported in these cells, including several mediators of NK cell activation (VAV3 and LYN) and a large array of NK receptors (KIR2DL3, KIR2DL4, KIR2DL1, KIR3DL1, KIR2DS5, etc.). In addition, we identified up to 161 genes that encode transcriptional regulators, some of unknown function in CD8+ T cells, that were differentially expressed in the course of differentiation. Overall, these results provide new insights into the regulatory networks involved in memory CD8+ T cell maintenance and T cell terminal differentiation.

2016-11-15 | GSE83157 | GEO

Epigenetic regulation of the transcriptional program in memory and terminally differentiated CD8+ T cells [HCAFIS_12_Gene_Expression]

2016-11-15 | GSE83158 | GEO

Epigenetic regulation of the transcriptional program in memory and terminally differentiated CD8+ T cells [HCAFIS_05_Methylation]

2016-11-15 | GSE83156 | GEO

Epigenetic regulation of the transcriptional program in memory and terminally differentiated CD8+ T cells [HCAFIS_03_ChIPseq]

2016-11-15 | GSE83155 | GEO