Dataset Information


Sustained R848 treatment of Mice

ABSTRACT: PCR Array Profiling - R848 does not induce TLR-signaling related genes in TLR7-/- mice. Aim: To corroborate TLR7-dependency of R848 in mice "Triggering TLR7 in mice induces immune activation and lymphoid system disruption, resembling HIV-mediated pathology", Baenziger et al. Blood 2008 Overall design: Mice (n=3/sample) were treated with 1mg/kg/d R848 or PBS (Mock) for 7 days. Thereafter, spleen was removed, mRNA extracted from whole splenic tissue and subsequently subjected to microarray RT² Profiler PCR array (Bioscience Corporation) on a Biorad iCycler. The PCR Array system examines the expression of 84 genes related to TLR-mediated signal transduction. Raw Data (i.e., Ct values) are shown. Ct values >35 or blank was set to 35. The supplementary file show data generated by real-time quantitative PCR normalized to Hypoxanthine guanine phosphoribosyl transferase 1, Heat shock protein 90kDa alpha, and Glyceraldehyde-3-phosphate dehydrogenase, displayed as fold difference in the expression of each gene between R848- and Mock-treated samples. Two independent experiments are summarized for wild-type mice. TLR7- and IFNAR-/- animals were tested once.

INSTRUMENT(S): Mouse Toll-Like Receptor Signaling Pathway Microarray RT² Profiler™ PCR Array

SUBMITTER: Stefan Baenziger  

PROVIDER: GSE12957 | GEO | 2008-09-30



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Triggering TLR7 in mice induces immune activation and lymphoid system disruption, resembling HIV-mediated pathology.

Baenziger Stefan S   Heikenwalder Mathias M   Johansen Pål P   Schlaepfer Erika E   Hofer Ursula U   Miller Regina C RC   Diemand Simone S   Honda Kenya K   Kundig Thomas M TM   Aguzzi Adriano A   Speck Roberto F RF  

Blood 20080929 2

Chronic immune activation is a major cause for progressive immunodeficiency in human immunodeficiency virus type-1 (HIV) infection. The underlying trigger, however, remains largely unknown. HIV single-stranded RNA is a potent immune activator by triggering Toll-like receptor (TLR) 7/8. Thus, we hypothesized that sustained TLR7 triggering induces chronic immune activation and thereby contributes to progressive immunodeficiency. We used the synthetic compound R848 or a mixture of uridine-rich HIV  ...[more]

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