Transcriptomics

Dataset Information

32

Protective response to Brucella infection


ABSTRACT: Brucella ovis causes an important disease characterized by decreased fertility in rams, sporadic abortions in ewes and increased lamb mortality. The live attenuated B. melitensis Rev.1 vaccine is considered the best vaccine available against this infection. However, this vaccine shows variable protective efficacy ranging from 40% to 100%.The objective of this study was to identify possible correlates of protective response to B. ovis infection through the characterization by microarray hybridization and real-time RT-PCR of inflammatory and immune response genes upregulated in rams previously immunized with the B. melitensis Rev 1 vaccine strain and experimentally challenged with B. ovis. Gene expression profiles were compared before and after challenge with B. ovis between rams protected and those vaccinated but found infected after challenge. The genes upregulated in vaccinated and protected rams provide possible correlates of protective response to B. ovis infection in rams immunized with the B. melitensis Rev 1 vaccine. Overall design: Gene expression profiles were compared before and after vaccination and challenge with B. ovis between rams protected with the B. melitensis Rev 1 vaccine strain and those vaccinated but infected after challenge. Total RNA was isolated from buffy coat samples before vaccination (T0), after vaccination and before challenge (T1) and 60 days post challenge (T2) using TriReagent (Sigma, St. Louis, MO, USA)

INSTRUMENT(S): OSU RIGUA (Ruminant Immuno-inflammatory Gene Universal Array)

ORGANISM(S): Ovis aries  

SUBMITTER: Ruth Cecilia Galindo 

PROVIDER: GSE13202 | GEO | 2008-10-17

SECONDARY ACCESSION(S): PRJNA109503

REPOSITORIES: GEO

Similar Datasets

2010-06-25 | E-GEOD-13202 | ArrayExpress
2010-06-28 | GSE21149 | GEO
2010-07-29 | E-GEOD-21149 | ArrayExpress
2012-05-30 | E-GEOD-33251 | ArrayExpress
2009-09-30 | GSE18323 | GEO
2009-10-10 | E-GEOD-18323 | ArrayExpress
2014-10-01 | E-GEOD-51839 | ArrayExpress
| GSE26808 | GEO
2012-02-08 | GSE35612 | GEO
2014-09-26 | E-GEOD-61766 | ArrayExpress