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Massively parallel splicing reporter assay on designed sequence libraries


ABSTRACT: We designed 4 oligonucleotide libraries containing either a retained intron, a cassette exon, tandem 5' or tandem 3' splice sites, cloned them into dedicated reporter constructs, transfected and integrated these constructs in the genome of K562 cells, and performed targeted RNA sequencing to determine RNA splicing ratios and a FACSseq approach to determine protein isoform ratios. Overall design: Massively parallel reporter assay for alternative splicing

INSTRUMENT(S): Illumina NextSeq 500 (Homo sapiens; synthetic construct)

SUBMITTER: Martin Mikl 

PROVIDER: GSE132064 | GEO | 2019-06-03

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
GSE132064_casdf.csv.gz Csv
GSE132064_fivedf.csv.gz Csv
GSE132064_irdf.csv.gz Csv
GSE132064_threedf.csv.gz Csv
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