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Massively parallel reporter assay for programmed ribosomal frameshifting on designed sequence libraries


ABSTRACT: We designed an oligonucleotide libraries containing a potential frameshifting site, cloned the library variants into a dedicated reporter construct, transfected and integrated these constructs in the genome of K562 cells, and performed FACSseq (as well as targeted RNA sequencing) to determine if and to what extent frameshifting occurs. Overall design: Massively parallel reporter assay for programmed ribosomal frameshifting

INSTRUMENT(S): Illumina NextSeq 500 (Homo sapiens)

ORGANISM(S): Homo Sapiens

SUBMITTER: Martin Mikl 

PROVIDER: GSE145684 | GEO | 2020-02-22

REPOSITORIES: GEO

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