Dataset Information


Expression profiles of beta-hematin (BH)- or 4-hydroxy-2-nonenal (HNE)-treated RAW 264.7 cells

ABSTRACT: Hemozoin phagocytosis results in immunomodulation. In the present study, gene expression profiles were analyzed to identify transcriptional changes associated with two of the individual components of Hz in a model macrophage cell line. LPS-stimulated RAW 264.7 cells were exposed to the synthetic form of Hz, β-hematin (BH, 0.1 mg/mL) and HNE (35 μM) for 6 or 24 h. Keywords: variable treatment Overall design: Three biological replicates were analyzed for each 3 treatment types (HNE, BH, bead) in LPS stimulated cells in addition to 3 replicates of untreated LPS stimulated cells (control). Each replicate was composed of 6 pooled wells from a 6-well plate. In order to identify transcriptional changes dependent on interactions of BH rather than those due to phagocytosis, differentially expressed mRNAs were controlled by a corresponding particulate latex bead challenge.

INSTRUMENT(S): ABI Mouse Genome Survey Microarray

SUBMITTER: David W Wright  

PROVIDER: GSE13281 | GEO | 2009-10-16



altmetric image


Comparative analysis of gene expression changes mediated by individual constituents of hemozoin.

Schrimpe Alexandra C AC   Wright David W DW  

Chemical research in toxicology 20090301 3

Plasmodium protozoa, the source of malarial infections, catabolize large quantities of hemoglobin during an intraerythrocytic phase. During this process, free heme is detoxified through biomineralization into an insoluble heme aggregate, hemozoin (Hz). In its native state, Hz is associated with a variety of lipid peroxidation products including 4-hydroxy-2-nonenal (HNE). In the present study, gene expression profiles were used to compare responses to two of the individual components of Hz in a m  ...[more]

Similar Datasets

2009-12-17 | GSE15070 | GEO
2009-09-10 | GSE17761 | GEO
2010-05-05 | E-GEOD-17761 | ArrayExpress
2007-12-12 | GSE9859 | GEO
2007-09-29 | E-GEOD-935 | ArrayExpress
| GSE63889 | GEO
2019-02-28 | PXD012155 | Pride
2016-06-01 | E-GEOD-74060 | ArrayExpress
2016-02-25 | E-GEOD-76562 | ArrayExpress
2016-08-09 | PXD003335 | Pride