Transcriptomics,Genomics

Dataset Information

22

Expression profiles of beta-hematin (BH)- or 4-hydroxy-2-nonenal (HNE)-treated RAW 264.7 cells


ABSTRACT: Hemozoin phagocytosis results in immunomodulation. In the present study, gene expression profiles were analyzed to identify transcriptional changes associated with two of the individual components of Hz in a model macrophage cell line. LPS-stimulated RAW 264.7 cells were exposed to the synthetic form of Hz, β-hematin (BH, 0.1 mg/mL) and HNE (35 μM) for 6 or 24 h. Keywords: variable treatment Overall design: Three biological replicates were analyzed for each 3 treatment types (HNE, BH, bead) in LPS stimulated cells in addition to 3 replicates of untreated LPS stimulated cells (control). Each replicate was composed of 6 pooled wells from a 6-well plate. In order to identify transcriptional changes dependent on interactions of BH rather than those due to phagocytosis, differentially expressed mRNAs were controlled by a corresponding particulate latex bead challenge.

INSTRUMENT(S): ABI Mouse Genome Survey Microarray

SUBMITTER: David W Wright   

PROVIDER: GSE13281 | GEO | 2009-10-16

SECONDARY ACCESSION(S): PRJNA109601

REPOSITORIES: GEO

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Publications

Comparative analysis of gene expression changes mediated by individual constituents of hemozoin.

Schrimpe Alexandra C AC   Wright David W DW  

Chemical research in toxicology 20090301 3


Plasmodium protozoa, the source of malarial infections, catabolize large quantities of hemoglobin during an intraerythrocytic phase. During this process, free heme is detoxified through biomineralization into an insoluble heme aggregate, hemozoin (Hz). In its native state, Hz is associated with a variety of lipid peroxidation products including 4-hydroxy-2-nonenal (HNE). In the present study, gene expression profiles were used to compare responses to two of the individual components of Hz in a m  ...[more]

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