Genomics

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Single cell RNA sequencing analysis of CD8+ T cell subsets following DENV-vaccine administration


ABSTRACT: PBMC-derived CD8 T cell activation and gene expression profiles were assessed 14 days following a single dose of a candidate live-attenuated tetravalent denge virus vaccine (DENVax). Three distinct populations of CD8+ T cells were isolated by flow cytometric sorting: 1) CD3+ CD8+ CD38- HLA-DR- T cells, 2) CD3+ CD8+ CD38+ HLA-DR+ T cells, and 3) CD3+ CD8+ CD71+ HLA-DR+ T cells Overall design: Single cell RNA sequencing analysis of 3 different CD8 T cell populations present in a vaccine recpient 14 days post vaccine administration

INSTRUMENT(S): Illumina NextSeq 500 (Homo sapiens)

ORGANISM(S): Homo sapiens  

SUBMITTER: Adam Waickman  

PROVIDER: GSE132950 | GEO | 2019-08-05

REPOSITORIES: GEO

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