Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single-cell RNA-Sequencing data of posteriorized embryonic-like sacs, amniotic ectoderm-like cells, and H9 hESC

ABSTRACT: The study of early post-implantation human embryo development is limited by the scarcity of embryo samples and ethical restrictions. We developed a human pluripotent stem cell (hPSC)-based microfluidic platform to model several developmental processes during this stage. We applied single-cell mRNA sequencing technique to examine the transcriptomes of posteriorized embryonic-like sacs obtained using the microfluidic platform, as well as of amniotic ectoderm-like cells generated using a Transwell method and human embryonic stem cells (H9 hESCs).

ORGANISM(S): Homo sapiens

PROVIDER: GSE134571 | GEO | 2019/07/26

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Differentially Expressed Genes of Pseudomonas aeruginosa Isolates from Eyes with Keratitis and Healthy Conjunctival Sacs

Project description:P. aeruginosa isolates from keratitis and healthy conjunctival sacs were obtained. The transcriptome profile of P. aeruginosa was characterized by a high throughput RNA-seq strategy using the Illumina HiSeq 2500 platform. The DEGs were analyzed with DESeq and validated through quantitative real-time polymerase chain reaction (PCR) and with experimental mice.

2022-08-24 | GSE209539 | GEO

ON and OFF SACs identified by scRNA-seq

Project description:The goal of this experiment was to investigate the heterogeneity of SACs via scRNA-seq

2019-12-04 | GSE132507 | GEO

Comparing the cerebellar transcriptome of Sacs KO versus WT mice

Project description:Autosomal Recessive Spastic Ataxia of Charlevoix-Saguenay (ARSACS) is caused by mutations in SACS gene encoding sacsin. ARSACS patients and mouse models display early degeneration of cerebellum in agreement with high sacsin expression in this organ. We performed unbiased transcriptomic of cerebella from Sacs KO mice versus controls to dissect the mechanisms underlying cerebellar degeneration in ARSACS.

2023-08-08 | GSE200876 | GEO

Single-cell RNA-Sequencing data of posteriorized embryonic-like sacs, amniotic ectoderm-like cells, and H9 hESC

Project description:Single-cell RNA-Sequencing data of posteriorized embryonic-like sacs, amniotic ectoderm-like cells, and H9 hESC

| PRJNA555602 | ENA

RNA-sequencing of murine wild-type and Cdx-mutant E9.5 yolk sacs

Project description:E9.5 yolk sacs were collected from wild type (CD1) and Cdx-mutant (DKO) embryos and processed for RNA-sequencing to identify Cdx-dependent changes in gene expression

2022-02-01 | GSE181090 | GEO

Effects of Oct4 depletion on BM-related gene expression during the initial stages of post-implantation amniotic sac embryoid (PASE) development.

Project description:Basement membranes (BMs) are sheet-like structures of specialized extracellular matrix (ECM), which provide structural support for many tissues and play a central role in signalling. They are key regulators of cell behavior and tissue functions and defects in their assembly or composition are involved in numerous human diseases. Due to the differences between human and animal embryogenesis, ethical concerns, legal constraints, the scarcity of human tissue material and the inaccessibility of the in vivo condition, BM regulation during human embryo development remained elusive. Making use of a recently established embryoid allowed us to draw a picture of BM assembly during human embryogenesis and to examine BM disintegration during primitive streak (PS) cell dissemination. Further, we show that the transcription factor Oct4 regulates the expression of BM structural components and receptors and controls BM development during embryogenesis by regulating Akt signalling and the small GTPase Rac1. These results represent a relevant step towards a more comprehensive understanding of early human development. The deposited RNA-seq data aimed at evaluating the effects of siRNA-mediated depletion of the master transcription factor Oct4 on BM-related gene expression during initial stages of embryoid formation.

2024-03-06 | GSE255320 | GEO

Genome-wide analysis of gene expression of Flk1Cre;ETV2 CKO mouse yolk sacs.

Project description:To identify genes critical for vascular development, we generated mice where ETV2 is inactivated in FLK1+ cells by a loxP-Cre recombination approach. Results provide a detailed insight into the function of ETV2 in emrbyonic vasculare formation. Total RNA obtained from E9.5 yolk sacs from Flk1Cre;ETV2 CKO and control mice.

2014-11-17 | E-GEOD-51407 | biostudies-arrayexpress

RNA sequencing analysis of embryonic yolk sacs from wildtype and Etv2 mutant embryos at E8.5

Project description:Transcriptomic analyses of yolk sacs from mouse embryos at E8.5 was performed to assess the dosage dependent effects of varying Etv2 dosage on early endothelial and hematopoietic development.

2022-05-01 | GSE174546 | GEO

Single cell transcriptome sequencing of CS11 and CS15 human yolk sacs

Project description:Yolk sac is an important site for early embryonic hematopoiesis. However, our understanding of early hematopoietic development is still very limited. Single cell transcriptome sequencing provides us with a good research method. Here, we performed single cell RNA-seq analysis for Carnegie stage 11 (CS11) and Carnegie stage 15 (CS15) human yolk sacs.

2020-02-16 | GSE137010 | GEO

Zea mays Transcriptome or Gene expression

Project description:We perform a quantitative RNA-seq analysis of embryo sacs, comparator ovules with the embryo sacs removed, mature pollen, and seedlings to assist the identification of gametophyte functions in maize. Expression levels were determined for annotated genes in both gametophytes, and novel transcripts were identified from de novo assembly of RNA-seq reads. RNA-seq was performed on four tissue types: nine-day old, above-ground seedling (S); mature pollen (MP); embryo-sac-enriched samples with some remaining nucellar cells (ES); and ovules with embryo sacs removed (Ov).

2011-07-09 | E-MTAB-4128 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data