Genomics,Multiomics

Dataset Information

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Cdk1 controls global epigenetic landscape in embryonic stem cells


ABSTRACT: In this study, we found that in ES cells the majority of Cdk1 substrates are localized on chromatin. Cdk1 phosphorylates a large number of proteins involved in epigenetic regulation, including writers and erasers of all major histone marks. High levels of Cdk1 in ES cells phosphorylate and partially inactivate Dot1l, the histone H3 lysine 79 methyltransferase responsible for placing activating H3K79 marks on gene bodies. Decrease of Cdk1 activity during ES cell differentiation de-represses Dot1l, thereby allowing coordinated expression of differentiation genes. These analyses indicate that Cdk1 functions to maintain the epigenetic identity of ES cells. Overall design: In mouse embryonic stem cells: (1) Thio-ChIP-seq for the Cdk1 substrates-binding DNA; (2) H3K79me2 ChIP-seq upon Cdk1 inhibition; (3) Transcriptome of WT cells, and WT vs. Dot1l KO cells in SL medium and during Wnt3a and activin A-induced differentiation.

OTHER RELATED OMICS DATASETS IN: PXD015173

INSTRUMENT(S): Illumina HiSeq 2000 (Mus musculus)

ORGANISM(S): Mus musculus  

SUBMITTER: Chen Chu  

PROVIDER: GSE134645 | GEO | 2020-04-04

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
GSE134645_Dot1l_KO_LIF.csv.gz Csv
GSE134645_Dot1l_KO_Wnt.csv.gz Csv
GSE134645_H3K79me2_3MB_Peaks.bed.gz Bed
GSE134645_H3K79me2_DMSO_Peaks.bed.gz Bed
GSE134645_ThioChIP_Peaks.bed.gz Bed
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Publications


The cyclin-dependent kinase 1 (Cdk1) drives cell division. To uncover additional functions of Cdk1, we generated knockin mice expressing an analog-sensitive version of Cdk1 in place of wild-type Cdk1. In our study, we focused on embryonic stem cells (ESCs), because this cell type displays particularly high Cdk1 activity. We found that in ESCs, a large fraction of Cdk1 substrates is localized on chromatin. Cdk1 phosphorylates many proteins involved in epigenetic regulation, including writers and  ...[more]

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