Genomics

Dataset Information

0

Mild, moderate and severe enterovirus 71 (EV71) isolates infection in human PBMCs


ABSTRACT: Hand, foot and mouth disease (HFMD), caused by enterovirus 71 (EV71), presents mild to severe disease, and sometimes fatal neurological and respiratory manifestations. However, reasons for the severe pathogenesis remain undefined. To investigate this, infection and viral kinetics of EV71 isolates from clinical disease (mild, moderate and severe) from Sarawak, Malaysia, were characterized in human rhabdomyosarcoma (RD), neuroblastoma (SH-SY5Y) and peripheral blood mononuclear cells (PBMCs). High resolution transcriptomics was used to decipher EV71-host interactions in PBMCs. Ingenuity analyses revealed similar pathways triggered by all EV71 isolates, although the extent of activation varied. Importantly, several pathways were found to be specific to the severe isolate, including triggering receptor expressed on myeloid cells 1 (TREM-1) signaling. Depletion of TREM-1 in EV71-infected PBMCs with peptide LP17 resulted in decreased levels of pro-inflammatory genes, and reduced viral loads for the moderate and severe isolates. Mechanistically, this is the first report describing the transcriptome profiles during EV71 infections in primary human cells, and the involvement of TREM-1 in the severe disease pathogenesis, thus providing new insights for future treatment targets. Overall design: PBMCs from 4 donors were used. PBMCs were infected with three different viral isolates of EV71, and harvested at time points 0, 6, 12 and 24 hours post-infection. Mock-infected and heat-inactivated EV71 isolate were used as negative controls.

INSTRUMENT(S): Illumina HiSeq 2500 (Homo sapiens)

ORGANISM(S): Homo Sapiens

SUBMITTER: Siti Naqiah Amrun  

PROVIDER: GSE135964 | GEO | 2020-03-16

REPOSITORIES: GEO

altmetric image

Publications

TREM-1 activation is a potential key regulator in driving severe pathogenesis of enterovirus A71 infection.

Amrun Siti Naqiah SN   Tan Jeslin J L JJL   Rickett Natasha Y NY   Cox Jonathan A JA   Lee Bernett B   Griffiths Michael J MJ   Solomon Tom T   Perera David D   Ooi Mong How MH   Hiscox Julian A JA   Ng Lisa F P LFP  

Scientific reports 20200302 1


Hand, foot and mouth disease (HFMD), caused by enterovirus A71 (EV-A71), presents mild to severe disease, and sometimes fatal neurological and respiratory manifestations. However, reasons for the severe pathogenesis remain undefined. To investigate this, infection and viral kinetics of EV-A71 isolates from clinical disease (mild, moderate and severe) from Sarawak, Malaysia, were characterised in human rhabdomyosarcoma (RD), neuroblastoma (SH-SY5Y) and peripheral blood mononuclear cells (PBMCs).  ...[more]

Similar Datasets

| S-EPMC7052206 | BioStudies
| PRJNA560788 | ENA
| S-EPMC7092854 | BioStudies
| S-EPMC3212826 | BioStudies
| S-EPMC7935532 | BioStudies
| S-EPMC5707558 | BioStudies
| S-EPMC3223501 | BioStudies
| S-EPMC8765647 | BioStudies
| S-EPMC6668393 | BioStudies
| S-EPMC3315555 | BioStudies