Dataset Information


Transcriptome analyses of 2.5% and 5% NaCl-stressed B. cereus ATCC 14579

ABSTRACT: Bacteria are able to cope with the challenges of sudden increase of salinity by activating adaptation mechanisms. In this study, exponentially growing cells of the food-borne pathogen Bacillus cereus ATCC 14579 were exposed to both mild (2.5% NaCl w/v) and severe (5% NaCl w/v) salt stress conditions. B. cereus continued growth at a reduced rate when shifted to mild salt stress. Exposure to severe salt stress resulted in a lag period, and after 60 min cellular growth was resumed filamentously. Whole-genome expression analyses of cells exposed to 2.5% salt stress revealed an overlap with that of cells exposed to 5% salt stress, suggesting that the corresponding genes (n = 147) were involved in a general salt stress response. Up-regulation of osmolyte, Na+/H+ and di-/tripeptide transporters and activation of an oxidative stress response were important aspects of this general salt stress response. Activation of this response may confer cross-protection towards other stresses, and increased resistance to heat and H2O2 was indeed observed. Notably, a temporal shift was observed between the observed transcriptome and phenotype responses of severely salt-stressed cells including cellular filamentation, reduced chemotaxis performance, catalase activity and optimal oxidative stress resistance. The linkage of transcriptomes and phenotypic characteristics can contribute to a better understanding of cellular stress adaptation strategies and possible cross protection mechanisms. Overall design: ATCC 14579 was grown to an OD600 of ~0.5, after that 2.5% or 5% NaCl (w/v) was added. Before addition of NaCl and after 10, 30 and 60 minutes of NaCl-exposure a sample was taken for RNA isolation. Comparisons (untreated versus salt-treated) performed were in duplicate with dye swap.

INSTRUMENT(S): Bacillus cereus ATCC 14579, 2nd design, Agilent 2X11K

ORGANISM(S): Bacillus cereus ATCC 14579  

SUBMITTER: Heidy den Besten 

PROVIDER: GSE13713 | GEO | 2009-06-22



Similar Datasets

2009-01-01 | S-EPMC2698350 | BioStudies
2010-06-24 | E-GEOD-13713 | ArrayExpress
2019-01-01 | S-EPMC6784087 | BioStudies
2015-05-12 | E-GEOD-68767 | BioStudies
2016-01-01 | S-EPMC5010206 | BioStudies
2004-01-01 | S-EPMC524882 | BioStudies
2008-05-11 | GSE9855 | GEO
2008-05-11 | GSE9856 | GEO
2008-05-11 | GSE9858 | GEO
2008-01-01 | S-EPMC2358912 | BioStudies