Genomics

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Genome-wide expression analysis of memory-like inflammatory responses in murine micoglial cells (BV-2 immortalized microglial cell line)


ABSTRACT: Trained immunity and immune tolerance have been identified as long-term response patterns of the innate immune system. The causes of these opposing reactions remain elusive. Here we report about differential inflammatory responses of microglial cells derived from neonatal mouse brain to increasing doses of the endotoxin LPS. Prolonged priming with ultra-low LPS doses provokes trained immunity, i.e. increased production of pro-inflammatory mediators in comparison to the unprimed control. In contrast, priming with high doses of LPS induces immune tolerance implying decreased production of inflammatory mediators and pronounced release of anti-inflammatory cytokines. Investigation of the signaling processes and cell functions involved in these memory-like immune responses reveals essential role of phosphoinositide 3-kinase γ (PI3Kγ), one of the phosphoinositide 3-kinase species highly expressed in innate immune cells. Together, our data suggest profound influence of preceding contacts with pathogens on the immune response of microglia. The impact of these interactions – trained immunity or immune tolerance - appears to be shaped by pathogen dose. Overall design: Samples of murin microglial cells were analysed after one or two time treatment with LPS (12 samples: 3 treated once with LPS, 3 treated twice with LPS (high-dose), 3 treated twice with LPS (low-dose))

INSTRUMENT(S): Illumina HiSeq 2500 (Mus musculus)

ORGANISM(S): Mus musculus  

SUBMITTER: Marco Groth  

PROVIDER: GSE137741 | GEO | 2019-12-05

REPOSITORIES: GEO

Dataset's files

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GSE137741_count_RPMs_RPKMs.xls.gz Xls
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Publications

Memory-Like Inflammatory Responses of Microglia to Rising Doses of LPS: Key Role of PI3Kγ.

Lajqi Trim T   Lang Guang-Ping GP   Haas Fabienne F   Williams David L DL   Hudalla Hannes H   Bauer Michael M   Groth Marco M   Wetzker Reinhard R   Bauer Reinhard R  

Frontiers in immunology 20191108


Trained immunity and immune tolerance have been identified as long-term response patterns of the innate immune system. The causes of these opposing reactions remain elusive. Here, we report about differential inflammatory responses of microglial cells derived from neonatal mouse brain to increasing doses of the endotoxin LPS. Prolonged priming with ultra-low LPS doses provokes trained immunity, i.e., increased production of pro-inflammatory mediators in comparison to the unprimed control. In con  ...[more]

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