CRISPR/Cas9 in vivo screens in patients’ leukemias growing in mice

ABSTRACT: Functional genomic screens were mostly performed in cell lines and mouse models so far. To approximate the clinical situation, we established customized CRISPR/Cas9 screens in patient-derived xenograft (PDX) leukemia models in vivo. Ninety-two surface molecules were tested; sgRNAs against CXCR4 and ITGB1 dropped out and single knockouts validated their essential function. Our method allows determining patient-specific tumor dependencies in vivo which might facilitate personalized medicine of cancer in the future.

ORGANISM(S): Homo sapiens

PROVIDER: GSE139553 | GEO | 2023/07/12

REPOSITORIES: GEO