Transcriptomics

Dataset Information

46

RNA pol II ChIP on chip (RPCC)


ABSTRACT: Adaptation of the ChIP-on-chip protocol, to calculate genomic transcription rates in S. cerevisiae. Keywords: ChIP-chip Overall design: There are 3 different experimental conditions: -Yeast cell growing exponentially in YPD. -Yeast cell stopped after 2 h of changing them to YPGal. -Yeast cell growing exponentially after 14.5 h of changing them to YPGal. We have used 3 different IP protocols: -total RNA pol II using a Myc tagged RNA pol (RPB1-Myc) -RNA pol II CTD using the Ab 8WG16 (Covance) -RNA pol II CTD Phosphorylated on Ser5 (David Bentley\'s lab) There are 3 independent biological replicates of each experiment.

INSTRUMENT(S): Valencia yeast v6

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Jose E. Perez-Ortin  

PROVIDER: GSE14060 | GEO | 2009-08-24

SECONDARY ACCESSION(S): PRJNA114527

REPOSITORIES: GEO

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