Genomics

Dataset Information

46

ChIP-chip in RNA Pol II mutants


ABSTRACT: The carboxyl-terminal domain (CTD) of the largest subunit of RNA polymerase II (Pol II) orchestrates dynamic recruitment of specific cellular machines during different stages of transcription. Signature phosphorylation patterns of Y1S2P3T4S5P6S7 heptapeptide repeats of the CTD engage specific “readers.” While phospho-Ser5 and phospho-Ser2 marks are ubiquitous, phospho-Thr4 is reported to only impact specific genes. Here, we investigate the genome-wide occupancy of Pol II, phospho-Thr4, and key reader Rtt103 in WT and CTD-mutant strains of S. cerevisiae. Overall design: ChIP of Rpb3, phospho-Thr4, and Rtt103 in WT or CTD-mutant strains of S. cerevisiae.

INSTRUMENT(S): S. cerevisiae 120807_SCer_Dwolbach_CGH

SUBMITTER: Aseem Ansari 

PROVIDER: GSE95418 | GEO | 2017-05-02

SECONDARY ACCESSION(S): PRJNA377089

REPOSITORIES: GEO

altmetric image

Publications

Different phosphoisoforms of RNA polymerase II engage the Rtt103 termination factor in a structurally analogous manner.

Nemec Corey M CM   Yang Fan F   Gilmore Joshua M JM   Hintermair Corinna C   Ho Yi-Hsuan YH   Tseng Sandra C SC   Heidemann Martin M   Zhang Ying Y   Florens Laurence L   Gasch Audrey P AP   Eick Dirk D   Washburn Michael P MP   Varani Gabriele G   Ansari Aseem Z AZ  

Proceedings of the National Academy of Sciences of the United States of America 20170502 20


The carboxyl-terminal domain (CTD) of the largest subunit of RNA polymerase II (Pol II) orchestrates dynamic recruitment of specific cellular machines during different stages of transcription. Signature phosphorylation patterns of Y1S2P3T4S5P6S7 heptapeptide repeats of the CTD engage specific "readers." Whereas phospho-Ser5 and phospho-Ser2 marks are ubiquitous, phospho-Thr4 is reported to only impact specific genes. Here, we identify a role for phospho-Thr4 in transcription termination at nonco  ...[more]

Similar Datasets

| GSE95417 | GEO
| GSE97827 | GEO
2014-09-25 | E-GEOD-61713 | ArrayExpress
2013-12-16 | E-MTAB-1528 | ArrayExpress
2014-11-24 | E-GEOD-62519 | ArrayExpress
2015-04-02 | E-GEOD-67483 | ArrayExpress
2010-09-22 | E-MEXP-2850 | ArrayExpress
| GSE68484 | GEO
2010-05-20 | E-GEOD-21917 | ArrayExpress
2010-05-20 | GSE21917 | GEO