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Next Generation Sequencing Facilitates Quantitative Analysis of IFN-γ induced-MMP2/9 inhibition compared to vehicle control cells' Transcriptomes

ABSTRACT: Expression profiles of SB-3CT treated A375 human melanoma cell lines and vehicle control group with or without induced by IFN-γ.

ORGANISM(S): Homo sapiens

PROVIDER: GSE141585 | GEO | 2020/09/03

REPOSITORIES: GEO

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Next Generation Sequencing Facilitates Quantitative Analysis of IFN-γ induced-MMP2/9 inhibition compared to vehicle control cells' Transcriptomes

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Expression data from BRAFV600E A375 melanoma cells treated with vehicle or vemurafenib

Project description:Vemurafenib is a BRAF inhibitor with specificity for the most common BRAF mutant encountered in melanomas (BRAFV600E). Vemurafenib suppresses the proliferation of BRAF mutant human melanoma cells by suppressing downstream activation of the MEK/ERK mitogen activated protein kinases. We used microarrays to examine the transcriptional response of a vemurafenib-sensitive BRAFV600E human melanoma cell line (A375) to vemurafenib in order to further delineate the mechanisms by which BRAFV600E drives cell proliferation and energy metabolism in human melanoma. BRAFV600E A375 human melanoma cells were treated with vehicle (0.1% DMSO) or 10 uM vemurafenib for 24 h after which total RNA was extracted. Cells were prepared and RNA was extracted in 3 separate batches (three different cell stocks on three separate days) providing three independent replicates (n=3). Paired replicates (prepared from the same stock of cells on the same day) are denoted by A, B and C.

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