Transcriptomics,Genomics

Dataset Information

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MicroRNA profiling in the aqueous humor of highly myopic eyes by next generation sequencing


ABSTRACT: Purpose: To characterize microRNAs (miRNAs) and their possible roles in high myopia by using next generation sequencing Methods: Aqueous humor samples were obtained from 15 highly myopic eyes and 15 cataract eyes at the onset of surgery. miRNA next generation sequencing and bioinformatics analyses were performed using RNA extracted from aqueous humor samples. Results: A total of 341 miRNAs were detected in the aqueous humor samples of highly myopic eyes; 201 miRNAs were detected in the aqueous humor samples of cataractous control eyes. A total of 249 mature miRNAs and 17 novel miRNAs were differentially expressed during myopia. Possible pathways regulated by these aberrantly expressed miRNAs included the TNF, MAPK, PI3K-Akt, and HIF-1 signaling pathways. Conclusions: The current study provided an overall view of miRNA profiling in the aqueous humor of highly myopic eyes. These profiles may be associated with myopia pathogenesis, and are potential biomarkers. Overall design: miRNA profiles in aqueous humor of highly myopic patients (15 eyes of 15 patients), aqueous humor of cataract patients as control (15 eyes of 15 patients) , aquous humor from 3 eyes mixed as 1 sample, detected in quintuplicate, using Illumina Hiseq4000

INSTRUMENT(S): Illumina HiSeq 4000 (Homo sapiens)

ORGANISM(S): Homo sapiens  

SUBMITTER: Jibo Zhou  

PROVIDER: GSE142359 | GEO | 2021-12-15

REPOSITORIES: GEO

Dataset's files

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GSE142359_miRNA_Expression.xlsx Xlsx
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