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Genes induced by IL-9 in the colon of transgenic mice

ABSTRACT: The aim of this work was to identify genes induced by IL-9 in the colon of IL-9-tarnsgenic mice (Tg5). Therefore, we performed a comprehensive study of the genes expressed in the colon of IL-9 transgenic and wild type FVB mice, taking advantage of the affymetrix microarray technology. Keywords: cytokine-induced response

ORGANISM(S): Mus musculus

PROVIDER: GSE14249 | GEO | 2009/12/25

SECONDARY ACCESSION(S): PRJNA111177

REPOSITORIES: GEO

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Genes induced by IL-9 in the colon of transgenic mice

Project description:The aim of this work was to identify genes induced by IL-9 in the colon of IL-9-tarnsgenic mice (Tg5). Therefore, we performed a comprehensive study of the genes expressed in the colon of IL-9 transgenic and wild type FVB mice, taking advantage of the affymetrix microarray technology. Keywords: cytokine-induced response RNA was extracted from a piece of the distal colon from IL-9 transgenic or wild type TVB mice. RNA samples from 4 different mice were pooled for each group before hybridization on Affymetrix microarrays.

2009-12-24 | E-GEOD-14249 | biostudies-arrayexpress

IL-22 Prevents Epithelial Dysfunction During Citrobacter rodentium-induced Infectious Colitis

Project description:IBD is a complex autoimmune disease characterized by dysregulated interactions between host immune responses and microbiome at the intestinal epithelium interface. Here we identified shared protein alterations in intestinal epithelial differentiation and function between IBD and Citrobacter rodentium infected FVB mice. We discovered that prophylactic treatment with the mucosal healing therapy IL-22.Fc in the infected FVB mice reduced disease severity and rescued the mice from lethality. Notably, we observed an emergence of intermediate undifferentiated intestinal epithelial cells upon infection, with disrupted expression of the solute transporter machinery as well as components critical for intestinal barrier integrity. Multi-omics analyses revealed that with IL-22.Fc treatment several disease associated changes were prevented (including disruption of the solute transporter machinery), and proper physiological homeostatic functions of the intestine was restored. Taken together, we unveiled the disease relevance of the C. rodentium induced colitis model to IBD and demonstrated the protective role of the mucosal healing therapy IL-22.Fc in ameliorating the epithelial dysfunction.

2023-10-05 | PXD024688 | Pride

Murine colon: IL-10-/- versus BALB/c

Project description:MicroRNA profiling of mouse colonic tissue comparing severe colon pathology with mild and no pathology. Three condition experiment: BALB/c (No Pathology Control), IL-10-/- with Pathology = 1, IL-10-/- with Pathology > 3. Biological replicates were pooled RNA samples of 3 separate mice per condition.

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RNA-seq of mouse colon tissue treated with engineered IL-22 variants

Project description:RNA-seq analysis of colon tissue from mice treated with PBS, WT IL-22, or engineered IL-22 variant 22-B3.

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Gene expression data of human normal colon organoids stimulated by IL-17A

Project description:With aging, normal human tissues experience expansion of somatic clones that carry cancer mutations. However, whether such clonal expansion exists in the non-neoplastic intestine remains unknown. Here, from whole exome sequencing of 76 clonal human colon organoids, we identify a unique pattern of somatic mutagenesis in the inflamed epithelium of ulcerative colitis patients. The affected epithelium accumulates somatic mutations in multiple IL-17 signaling-related genes, including NFKBIZ, ZC3H12A, and PIGR, which are rarely identified in colon cancer. Targeted sequencing validates pervasive spread of IL-17 signaling-related mutations. Unbiased CRISPR-based knockout screening in colon organoids illuminates mutation-mediated resistance to IL-17A-induced proapoptotic response. Some of these genetic mutations are known to exacerbate experimental colitis in mice, and somatic mutagenesis in human colon epithelium may be causally linked to the inflammatory process. Our findings highlight a unique genetic landscape adapting to a hostile microenvironment and its potential contribution to the pathogenesis of ulcerative colitis.

2019-10-21 | GSE127757 | GEO

Mouse colon tumors compared to normal colon

Project description:Label-free proteomics from colon tumors induced in C57BL/6J mice following exposure to azoxymethane plus dextran sodium sulfate. Normal colon was taken from age matched mice not exposed to AOM/DSS.

2024-06-03 | PXD042677 | JPOST Repository

Gene induction by Dex, IL-9 and CCL-1 in BW5147 mouse T cell lymphoma

Project description:The aim of this work was to determine the genes and mechanisms involved in IL-9- and CCL1-mediated protection against glucocorticoid-induced apoptosis. We made the hypothesis that the expression of the really critical mediators could be regulated in a reciprocal way by Dex on the one hand and anti-apoptotic cytokines such as IL-9 and CCL1 on the other hand.Therefore, we performed a comprehensive study of the genes expressed by BW5147 cells stimulated with or without Dex, IL-9 and CCL1, taking advantage of the Affymetrix microarray technology. Keywords: cytokine and dexamethasone stimulation

2011-05-25 | GSE13823 | GEO

Gene induction by Dex, IL-9 and CCL-1 in BW5147 mouse T cell lymphoma

2011-05-25 | E-GEOD-13823 | biostudies-arrayexpress

Expression profiles of colon macrophages from macrophage-specific E4BP4 transgenic mouse [ColonMac_RNA-seq]

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to evaluate the effects of macrophage-specific E4BP4 overexpression under c-fms gene promoter on colon macrophages during the recovery phase of Dextran Sodium Sulfate (DSS)-induced colitis. Methods: We generated transgenic mice (TG) with macrophage-specific E4BP4 overexpression. Colon macrophages were isolated at Zeitgeber Time (ZT) 0 from TG mice and WT littermates and total RNA was extracted. Colon macrophage RNA profiles were generated by deep sequencing for two groups with three mouse samples each. Results: There were significant differences between TG and WT mice. Conclusions: Colon macrophages from the E4BP4 transgenic mice during the recovery phase of DSS-induced colitis might be altered at the transcription level.

2024-03-31 | GSE183075 | GEO

RNAseq analysis of colon and ileum tissue from naive wild type, MR1-/- and IL-17A-/- mice

Project description:Determination of differentially expressed genes in the proximal colon and distal ileum tissue in MR1 and IL-17A deficiency at steady-state. Tissue from naïve mice was harvested, total RNA extracted and subjected to RNASeq analysis.

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