Transcriptomics,Genomics

Dataset Information

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Interferon inducible GBPs restrict Burkholderia thailandensis motility induced cell-cell fusion


ABSTRACT: Innate immunity responds to pathogens by producing alarm signals and activating pathways that make host cells inhospitable for pathogen replication. The intracellular bacterium Burkholderia thailandensis invades the cytosol, hijacks host actin, and induces cell fusion to spread to adjacent cells, forming multinucleated giant cells (MNGCs) which promotes bacterial replication. We show that type I interferon (IFN) restricts macrophage MNGC formation during B. thailandensis infection. Guanylate-binding proteins (GBPs) expressed downstream of type I IFN were required to restrict MNGC formation through inhibition of Arp2/3-dependent actin motility during infection. GTPase activity and the CAAX prenylation domain were required for GBP2 recruitment to B. thailandensis, which restricted bacterial actin polymerization required for MNGC formation. Consistent with in vitro macrophages, Gbp2-/- Gbp5-/-, GbpChr3-KO mice were more susceptible to intranasal infection with B. thailandensis than wildtype mice. Our findings reveal that IFN and GBPs play a critical role in restricting cell-cell fusion during infection Overall design: We used microarrays to gene expression profiles in bone marrow-derived macrophages isolated from wild-type and Ifnar1-/- mice after infection with Burkholderia thailandensis

INSTRUMENT(S): [Clariom_S_Mouse] Affymetrix Mouse Clariom S Array

ORGANISM(S): Mus musculus  

SUBMITTER: Geoffrey Neale  

PROVIDER: GSE143936 | GEO | 2021-08-23

REPOSITORIES: GEO

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Innate immunity responds to pathogens by producing alarm signals and activating pathways that make host cells inhospitable for pathogen replication. The intracellular bacterium Burkholderia thailandensis invades the cytosol, hijacks host actin, and induces cell fusion to spread to adjacent cells, forming multinucleated giant cells (MNGCs) which promote bacterial replication. We show that type I interferon (IFN) restricts macrophage MNGC formation during B. thailandensis infection. Guanylate-bind  ...[more]

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