Transcriptomics

Dataset Information

17

Loss of the actin remodeler Eps8 causes calorie restriction and improved metabolic status in mice


ABSTRACT: In a variety of organisms, including mammals, caloric restriction improves metabolic status and lowers the incidence of chronic-degenerative diseases, ultimately leading to increased lifespan. Here we show that knockout mice for Eps8, a regulator of actin dynamics, display reduced bodyweight, partial resistance to age- or diet-induced obesity, and overall improved metabolic status. We present evidence that these phenotypes, which are associated to increased lifespan in the Eps8 null mice, are due to caloric restriction. This, in turn, is caused by reduced intestinal fat absorption, due to altered morphogenesis of microvilli in intestinal enterocytes. In the nematode, genetic removal of Eps8, causes a microvillar phenotype, indistinguishable from that observed in mice, which leads to early larval lethality. By exploiting the nematode model system, we demonstrate here that the actin bundling activity of Eps8 is indispensable for viability and proper intestinal morphogenesis. This result links a precise molecular function of Eps8 to proper microvillar morphogenesis, and therefore to the phenotype of Eps8-null mice. Our results implicate actin dynamics in individual variations in bodyweight, metabolic status and longevity. Overall design: cDNA expression profiling of liver of 4 mutant EPS8 -/- animals versus pool of 4 wt animals. 8 hybridisation including 4 dye swap experiments

INSTRUMENT(S): GSF/IEG mouse 21K array (+RZPD)

ORGANISM(S): Mus musculus  

SUBMITTER: Martin Irmler  

PROVIDER: GSE14454 | GEO | 2010-03-09

SECONDARY ACCESSION(S): PRJNA111565

REPOSITORIES: GEO

altmetric image

Publications

Sorry, this publication's infomation has not been loaded in the Indexer, please go directly to PUBMED or Altmetric.

Similar Datasets

2014-05-01 | E-GEOD-14454 | ArrayExpress
2014-05-13 | E-GEOD-46895 | ArrayExpress
2012-07-17 | E-GEOD-38635 | ArrayExpress
| PRJNA87197 | ENA
| PRJNA111565 | ENA
| GSE93903 | GEO
| GSE106672 | GEO
| GSE97074 | GEO
| GSE71711 | GEO
2015-08-20 | E-GEOD-70857 | ArrayExpress