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High-throughput RNAi screening for genes essential for senecent cell viability

ABSTRACT: We carried out the analysis using human skin fibroblast HCA2 (MJ90) cells. Cells were induced to be senescent cells by treatment with nutlin3a + RO3306. The cells were infected with lentiviruses enocoding shRNA against human genes (DECIPHER Human Modules 1-3). As a result, we found some gene candidates involved in senecent cell viability.

ORGANISM(S): Homo sapiens

PROVIDER: GSE145414 | GEO | 2020/02/18

REPOSITORIES: GEO

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Transcriptome analysis among young and senescent fibroblasts

Project description:We carried out the analysis using human skin fibrobllast HCA2 (MJ90) cells. Cells were indcued to be senescent cells by treatment with RO3306 and Nutlin3a. The transcriptome analysis between young cells (uniduced cells) nd senescent cells revealed lonrf2, a ubiqutin E3 ligase, is one of the genes highly expressed in senescnet cells.

2023-07-20 | GSE179465 | GEO

Transcriptome analysis among senescent cells induced by various stimuli

Project description:We carried out the analysis using human skin fibrobllast HCA2 (MJ90) cells. Cells were indcued to be senescent cells by treatment with doxorubicin for 24 hrs, tert-butyl-hydroperoxide for 24 hrs, or serial passages as well as nultin3a + RO3306. As a result, we found simailar transcriptome signatures among senescent cells induced by various stimuli

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Transcriptome analysis among young and senescent fibroblasts expressing shLuciferase or shFbxo22

Project description:We carried out the analysis using human skin fibrobllast HCA2 (MJ90) cells dox-inducible shLuciferase or shFbxo22. Cells were indcued to be senescent cells by treatment with 10 Gy. As a result, Gene set enrichment analysis revealed a significant increase in the expression of lysomal-related and autophagosomal genes in control cells, whereas this gene ontology was not increased in cells depleted of Fbxo22

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Bulk RNA-seq of Normal and Doxorubicin-Induced Senescent HCA2 cells

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2022-08-27 | GSE198396 | GEO

Gene expression profiling of human neural crest cells and smooth muscle cells treated with DMSO or Nutlin3a

Project description:Activation of the p53 transcription factor triggers apoptosis only in certain cell types. Human neural crest cells (hNCCs), which can be generated from human embryonic stem cells, undergo apoptosis when treated with the p53 activating drug Nutlin3a. In contrast, when hNCCs are differentiated towards the smooth muscle cell lineage (early-hSMCS), they no longer undergo apoptosis in response to Nutlin3a treatment. Here, we sought to determine whether the transcriptional response to p53 activation differs between cells that are susceptible to p53-driven apoptosis and cells that are resistant to p53-driven apoptosis. To this end, we performed RNA-seq to assess the transcriptional response to Nutlin3a treatment in hNCCs and early-hSMCs.

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Expression level comparison under dividing and quiescent states in human primary fibroblasts

Project description:To identify genes with different expression levels in dividing and quiescent cells, mRNA-sequencing was done in both conditions for human primary fibroblasts (HCA2-hTert).

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RNA-seq following TBL1XR1 KD in human CD34+CD38- cord blood cells

Project description:We performed RNA-seq on CD34+CD38- human cord blood (CB) cells transduced with sh_Control or sh_TBL1XR1 lentiviruses to identify differentially expressed genes. Sorted CD34+CD38- cells from 3 independent cord blood pools were transduced with lentiviruses containing BFP reporter gene. BFP+ cells were sorted 3 days post-transduction and RNA was harvested for library preparation and sequencing.

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RNA-seq following miR-130a overexpression (OE) in human CD34+cord blood cells

Project description:We performed RNA-seq on CD34+ human cord blood (CB) cells transduced with Control or miR-130a OE lentiviruses to identify differentially expressed genes. Sorted CD34+ cells from 3 independent cord blood pools were transduced with lentiviruses containing mOrange (mO) reporter gene. mO+ cells were sorted 3 days post-transduction and RNA was harvested for library preparation and sequencing.

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A novel multiplex cell viability assay for high-throughput RNAi screening.

Project description: Not available

| S-EPMC3230607 | biostudies-literature

Comparison of gene expresson between larger sized tetraploid and smaller sized diploid HCA2

Project description:In order to determine the factors that regulate senescence induction, we tried to identfy genes that are predominantly expressed in large sized senescent cells with tetraploid DNA. We sorted large or small sized HCA2 cells treated wth IR (10Gy) according to size and structure (FSC and SSC) by FACScan and analyzed global gene expression patterns.

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