Dataset Information


ClpV3 affects pathogenicity in Pseudomonas aeruginosa

ABSTRACT: ClpV3 is a cytoplasmic AAA+ ATPase protein and is an essential component of H3-T6SS in Pseudomonas aeruginosa. Here, we report that an H3-T6SS deletion mutant PAO1(ΔclpV3) significantly affected the virulence-related phenotypes including pyocyanin production, biofilm formation, proteolytic activity and motilities. Most interestingly, the expression of T3SS genes was markedly affected, indicating a strong link between H3-T6SS and T3SS. RNA-Sequencing was performed to globally identify the genes differentially expressed when H3-T6SS was inactivated and the results obtained could be well correlated to the observed phenotypes. Overall design: Gene expression was compared between wild-type P. aeruginosa PAO1 and clpV3 deletion mutant.

INSTRUMENT(S): Illumina HiSeq 2500 (Pseudomonas aeruginosa PAO1)

ORGANISM(S): Pseudomonas aeruginosa PAO1  

SUBMITTER: Kangmin Duan  

PROVIDER: GSE148116 | GEO | 2020-04-20


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