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Kindlin-2 modulates PTH1R to regulate bone mass and PTH actions in bone

ABSTRACT: To investigation the role of PTH and Kindlin-2 in bone development, we performed single-cell RNA-sequencing. From Con-veh, Con-PTH, cKO-veh, cKO-PTH, we profiled more than 20k single cells, including multi-potent mesenchymal stromal cells (MSC), osteoprogenitors, osteoblasts, chondrocytes, fibroblasts, endothelial cells, smooth muscle cells, skeletal muscle cells, pericytes, and schwann cells. We found proportion of part of these cells were significant altered by PTH or Kindlin-2 loss, especially for MSC, osteoblast, chondrocyte, and fibroblast. Transcriptomic analysis revealed gene expression was dramatically regulated by PTH or Kindlin-2 loss.

ORGANISM(S): Mus musculus

PROVIDER: GSE150291 | GEO | 2020/10/01

REPOSITORIES: GEO

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Kindlin-2 modulates PTH1R to regulate bone mass and PTH actions in bone

Project description:Kindlin-2 modulates PTH1R to regulate bone mass and PTH actions in bone

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