Dataset Information


Peripheral blood transcriptomics_Human_Single supratherapeutic dose of acetaminophen

ABSTRACT: This was a controlled pilot study of 9 healthy adults admitted to the General Clinical Research Center at the University of North Carolina Hospital. After 3 days of clinical acclimatization, 6 subjects received a single 4g bolus dose or a placebo. Peripheral blood was collected on each day preceeding dosage, and then 6, 18, 24, 48, 72, and 96 hours post dosing. Total RNA was extracted and analyzed for differential gene expression on Agilent Human 1vA2 microarray chips. Overall design: 7.5 mls (2.5 ml in 3 PAXgene tubes) of blood were collected at each time point. Total RNA was amplified and labeled according to Agilent protocols and a single chip was run using a referenced design with Stratagenes Universal Human reference RNA. Each patient was normalized to there own 0 hr data. Significant differential expression was determined by comparing APAP treated subjects to placebos.

INSTRUMENT(S): Agilent-012097 Human 1A Microarray (V2) G4110B (Probe Name version)

SUBMITTER: NIEHS Microarray Core  

PROVIDER: GSE15158 | GEO | 2009-10-06



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Acetaminophen dosing of humans results in blood transcriptome and metabolome changes consistent with impaired oxidative phosphorylation.

Fannin Rick D RD   Russo Mark M   O'Connell Thomas M TM   Gerrish Kevin K   Winnike Jason H JH   Macdonald Jeffrey J   Newton Jack J   Malik Shahid S   Sieber Stella O SO   Parker Joel J   Shah Ruchir R   Zhou Tong T   Watkins Paul B PB   Paules Richard S RS  

Hepatology (Baltimore, Md.) 20100101 1

UNLABELLED:The diagnosis and management of drug-induced liver injury (DILI) is hindered by the limited utility of traditional clinical chemistries. It has recently been shown that hepatotoxicants can produce compound-specific changes in the peripheral blood (PB) transcriptome in rodents, suggesting that the blood transcriptome might provide new biomarkers of DILI. To investigate in humans, we used DNA microarrays as well as serum metabolomic methods to characterize changes in the transcriptome a  ...[more]

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