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Top-Down proteomics of unfractionated Bovine heart mitochondria

ABSTRACT: Top-Down proteomics pilot experiment of unfractionated Bovine Heart Mitochondria (BHM) using ultra high resolution Q-ToF tandem mass spectrometry (maXis 4G ETD, Bruker Daltonics).

INSTRUMENT(S):

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Heart

SUBMITTER: Hans Wessels

LAB HEAD: Alain J van Gool

PROVIDER: PXD005350 | Pride | 2018-04-05

REPOSITORIES: Pride

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			Action	DRS
	BHMMitoPLRPSTriversa10ul.mzXML	Mzxml
	DissectFeatures_BHM.xlsx	Xlsx
	TopPIC.zip	Other

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Publications

Towards a routine application of Top-Down approaches for label-free discovery workflows.

Schmit Pierre-Olivier PO Vialaret Jerome J Wessels Hans J C T HJCT van Gool Alain J AJ Lehmann Sylvain S Gabelle Audrey A Wood Jason J Bern Marshall M Paape Rainer R Suckau Detlev D Kruppa Gary G Hirtz Christophe C

Journal of proteomics 20170830

Thanks to proteomics investigations, our vision of the role of different protein isoforms in the pathophysiology of diseases has largely evolved. The idea that protein biomarkers like tau, amyloid peptides, ApoE, cystatin, or neurogranin are represented in body fluids as single species is obviously over-simplified, as most proteins are present in different isoforms and subjected to numerous processing and post-translational modifications. Measuring the intact mass of proteins by MS has the advan ...[more]

PMID: 28855124

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