Transcriptomics,Genomics

Dataset Information

19

Expression profiling of nasopharyngeal carcinoma cell lines


ABSTRACT: To identify the deregulated genes which may involved in the carcinogenesis of nasopharyngeal carcinoma (NPC). To identify potential tumor suppressor genes / oncogenes of NPC, we have employed whole genome microarray expression profiling as a discovery platform to analyze two NPC cell lines, TW01 and HONE1. For the purpose to filter out differential genes may not related to carcinogenesis of NPC, a gene expression database for premalignant nasopharyngeal epithelial cells was obtained from NP460hTert cells, and we used the commercial universal human RNA (stratagene) as common reference for all microarray experiments. A total of 1272 genes were commonly up-regulated (fold change > 2) and 1810 genes were commonly down-regulated (fold change < 0.5) in TW01 and HONE1 cell lines, whereas not deregulated in NP460hTert cells. Overall design: Four samples including two NPC cell lines (TW01 and HONE1), an immortalized premalignant epithelial cell line NP460hTert, and the commercial universal human RNA (common reference) were analyzed. Differential genes found in NP460hTert were not considered as NPC-related. Biological replicate experiments were performed for each sample.

INSTRUMENT(S): Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version)

SUBMITTER: Chia Huei Lee   

PROVIDER: GSE15170 | GEO | 2010-03-09

SECONDARY ACCESSION(S): PRJNA123123

REPOSITORIES: GEO

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Publications

Independent effect of EBV and cigarette smoking on nasopharyngeal carcinoma: a 20-year follow-up study on 9,622 males without family history in Taiwan.

Hsu Wan-Lun WL   Chen Jen-Yang JY   Chien Yin-Chu YC   Liu Mei-Ying MY   You San-Lin SL   Hsu Mow-Ming MM   Yang Czau-Siung CS   Chen Chien-Jen CJ  

Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology 20090331 4


This study aimed to assess independent effects of EBV and cigarette smoking on nasopharyngeal carcinoma, which have never been assessed in long-term follow-up studies. A cohort of 9,622 men was enrolled from 1984 to 1986. Blood samples collected at study entry were tested for antibodies against EBV antigens (anti-EBV) viral capsid antigen immunoglobulin A and DNase. The cigarette smoking habit was inquired through questionnaire interview. Newly developed nasopharyngeal carcinoma cases were ascer  ...[more]

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