Gut microbiota influences the expression of microRNA and their target mRNA in murine colonic lamina propria mononuclear cells [mRNA]
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ABSTRACT: Microarray analyses were carried out to compare expression profile of microRNA and mRNA in colonic lamina propria mononuclear cells between germ-free and SPF mice. Germ-free mice showed higher levels of some microRNAs and lower expression of target mRNAs, suggesting that microRNAs mediate gut microbiota regulation of gut immunity.
Project description:Microarray analyses were carried out to compare expression profile of microRNA and mRNA in colonic lamina propria mononuclear cells between germ-free and SPF mice. Germ-free mice showed higher levels of some microRNAs and lower expression of target mRNAs, suggesting that microRNAs mediate gut microbiota regulation of gut immunity.
Project description:To further development of our gene expression approach to CD300a deficiency on dendritic cells (DCs) in colonic lamina propria, we have employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential to distinguish CD300a deficiency on DCs in colonic lamina propria from those of WT mice. Colonic lamina propria DCs were obtained by cell sorter from WT and CD300a deficient mice raised under SPF and GF condition. Expression of Ifnb1 was significantly higher in CD300a deficient DCs, quantified in the same RNA samples by real-time PCR. Gene expression in WT and CD300a colonic lamina propria DCs raised under SPF and GF conditions were measured. Colonic lamina propria cells were obtained from 5 mice in each conditions. Takara-Bio
Project description:We analyzed the transcriptional profile of small-intestinal lamina propria (SI-LP) CD4+ T cells isolated from germ-free and mice monocolonized with Bifidobacterium adolescentis, SFB, and Nexabiotic (a 23-strain, Th17-inducing, probiotic mix).
Project description:The levels of some murine mRNAs and proteins are expressed discrepantly between the central immune system and the gut immune system in murine colitis. It was possible that RNA interference would result in some of the discrepancy. Thus, we compared microRNAs that are expressed in CD4+ T cells of spleen (SP) and lamina propria (LP) using Il-10+/- mice (C57BL/6 background) in order to reveal the role of miRNAs in murine colitis. Using 10-week-old female Il-10+/- mice, we investigated the difference in miRNA expressions between CD4+ T cells of spleen and that of lamina propria.
Project description:The goals of this study were to assess the differentially expressed transcripts in CD4+ cell isolated from the ileal lamina propria of germ-free (GF) and E. lenta strain 2243 monocolonized mice.
Project description:We report that Klebsiella pneumoniae promote Th1 cell induction in colon. To examine the influence of Klebsiella on colonic epithelial cells (ECs) and lamina propria CD11c+ dendritic cells (DCs), we performed RNA seq on them. Germ free mice were orally inoculated with Kp-2H7 or BAA-2552 and total RNA was isolated from colonic ECs and DCs 1 week after inoculation. Furthermore, we examined the involvement of TLRs in induction of Th1 cells using Myd88 KO, Trif KO, Myd88/Trif DKO mice. These deficient germ free mice were orally inoculated with Kp-2H7 and total RNA was isolated from colonic ECs 3 weeks after inoculation.
Project description:To further development of our gene expression approach to CD300a deficiency on dendritic cells (DCs) in colonic lamina propria, we have employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential to distinguish CD300a deficiency on DCs in colonic lamina propria from those of WT mice. Colonic lamina propria DCs were obtained by cell sorter from WT and CD300a deficient mice raised under SPF and GF condition. Expression of Ifnb1 was significantly higher in CD300a deficient DCs, quantified in the same RNA samples by real-time PCR.
Project description:The levels of some murine mRNAs and proteins are expressed discrepantly between the central immune system and the gut immune system in murine colitis. It was possible that RNA interference would result in some of the discrepancy. Thus, we compared microRNAs that are expressed in CD4+ T cells of spleen (SP) and lamina propria (LP) using Il-10+/- mice (C57BL/6 background) in order to reveal the role of miRNAs in murine colitis.
Project description:This experiment was investigating how gut commensal bacteria and intestinal inflammation affect miRNA expression. We analyzed miRNA expression of spleen and intestine from specific pathogen free (SPF) B6 mice, germ-free (GF) B6 mice, and IL-10 knockout mice which have severe colitis by microarray. Thus we have total 6 samples: GF spleen; GF intestines; SPF spleen; SPF intestine; IL-10 KO spleen and IL-10 KO intestine. We directly isolated RNA from whole spleens or intestines without any treatments, and then did microarray analysis.
Project description:Since the first findings that germ-free mice appeared resistant to diet-induced obesity, an increasing number of studies have emphasized the role of the gut microbiota as a core regulator of host weight gain and metabolism. However, several studies have not been replicable and conflicting results have left the discussion surrounding causality elusive. Specifically, the importance of early microbial dysbiosis in later development of obesity and metabolic disorders has been a subject of debate. Male Swiss Webster mice raised under germ-free conditions were introduced to a specific-pathogen-free (SPF) facility for microbial colonization at 1 week (1W) and 3 weeks (3W) of age. They were compared with control animals (SPF) with a complete gut microbiota from birth. Liver RNAseq of 7 week old animals showed differential gene expression between the groups