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Sperm circular RNAs: Types, expression patterns and epigenetic potential

ABSTRACT: Circular RNAs (circRNAs) are a widely distributed new type of non-coding RNAs with important potential regulatory roles. They are synthesized by backsplicing and lack the 3’-free end, which confer a higher stability and longer half-lives than linear RNAs. We aimed to characterize and experimentally validate human sperm circRNAs. Our results showed a complex and stable population of sperm exonic and intronic circRNAs. The RNase R resistance treatment test revealed three distinct specific expression patterns for sperm circRNAs. In contrast to circRNAs described in somatic cells, most of sperm circRNAs are mostly in their circular form without the presence of their linear cognates. Surprisingly, we also identified some circRNAs sensible to the RNase R treatment due to a non-stochastically decircularization. Sperm circRNAs arise mainly from genes enriched in epigenetic functions. Additionally, several previously reported sperm RNA elements required to achieve a live birth and/or altered in obese patients were found in the present study as being circRNAs. Likewise, some early embryonic circRNAs appear to be specifically provided by sperm and potentially translated to functional peptides. Altogether, these results suggest that sperm circRNAs could be crucial for fertility and early embryogenesis and could probably modulate some phenotype traits of the offspring.

ORGANISM(S): Homo sapiens

PROVIDER: GSE158778 | GEO | 2025/12/31

REPOSITORIES: GEO

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