Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Protein Phosphatase 1 regulates atypical chromosome segregation and cell polarity during mitotic and meiotic division in Plasmodium sexual stages

ABSTRACT: PP1 is a conserved serine/threonine phosphatase that regulates many aspects of mitosis and meiosis, often working in concert with other phosphatases, such as CDC14 and CDC25, in model eukaryotes. However, the malaria parasite, Plasmodium spp. lacks the CDC14 and CDC25 genes. The proliferative stages of the parasite life cycle include sexual development within the mosquito vector, with male gamete formation characterized by an atypical rapid mitosis, with three rounds of DNA synthesis, successive spindle formation with clustered kinetochore, and a meiotic stage during zygote to ookinete development following fertilization. It is unclear how PP1 is involved these unusual processes. Using real-time live-cell imaging, conditional gene knockdown, RNAseq and proteomic approaches, we show that Plasmodium PP1 is involved in both chromosome segregation during mitotic exit, and establishment of cell polarity during these sexual stages, suggesting that PP1 inhibitors may block parasite transmission.

ORGANISM(S): Plasmodium berghei

PROVIDER: GSE164175 | GEO | 2021/07/08

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Protein Phosphatase 1 regulates atypical chromosome segregation and cell polarity during mitotic and meiotic division in Plasmodium sexual stages

Project description:PP1 is a conserved eukaryotic serine/threonine phosphatase that regulates many aspects of mitosis and meiosis, often working in concert with other phosphatases, such as CDC14 and CDC25. The proliferative stages of the parasite life cycle include sexual development within the mosquito vector, with male gamete formation characterized by an atypical rapid mitosis, consisting of three rounds of DNA synthesis, successive spindle formation with clustered kinetochores, and a meiotic stage during zygote to ookinete development following fertilization. It is unclear how PP1 is involved in these unusual processes. Using real-time live-cell imaging, conditional gene knockdown, RNA-seq and proteomic approaches, we show that Plasmodium PP1 is involved in both chromosome segregation during mitotic exit, and potentially establishment of cell polarity during zygote development in the mosquito midgut, suggesting that chemical inhibitors of PP1 may be explored for blocking parasite transmission

2021-09-10 | PXD023571 | Pride

Plasmodium berghei Leucin Rich Repeat 1 involvement in early sporogony

Project description:Phosphatase type 1 is a major enzyme essential to Plasmodium development. However, the detailed mechanisms underlying its regulation remain to be deciphered. In this work, we report the functional characterization of the Plasmodium berghei LRR1, an ortholog of SDS22, one of the most ancient and conserved Phosphatase type 1 (PP1) interactor. SDS22 has been described as a PP1 regulator essential to critical cellular features such as motility, polarity, epithelium integrity or mitosis completion. Our study shows that in Plasmodium berghei, PbLRR1 is expressed during the intra-erythrocytic developmental cycle and up to the zygote stage during sexual development. PbLRR1 can be found in complex with PbPP1 in both asexual and sexual stages and is able to inhibit the phosphatase activity. Then, genetic analysis demonstrated that PbLRR1 deletion affects the course of the parasite development during early sporogony which manifest by the production of fewer and smaller oocysts. Finally, PbLRR1 interactome analysis associated with phospho-proteomics studies led to the identification of several putative PbLRR1/PbPP1 substrates. Although previously unsuspected PP1 substrates, some of them have been characterized as essential to the parasite sexual development. In addition, and for the first time, we identify the PP1 inhibitor 3 as a substrate of the PP1/LRR1 complex. In summary, this study provides insights into previously unrecognized PbPP1 fine regulation of Plasmodium early sporogony development through its interaction with PbLRR1.

2022-10-14 | PXD030389 | Pride

Genome-wide RIP-Chip analysis of translational repressor-bound mRNAs in the Plasmodium gametocyte

Project description:Following fertilisation, translation of stored, but previously translationally repressed transcripts, is instrumental in shaping the proteome of the early embryo of multicellular eukaryotes; further development relies on de novo transcription of the zygotic genome. A related process was evidenced in the unicellular protozoa Plasmodium berghei during transmission to the mosquito host where fertilisation and zygote development result in the formation of an entirely new cell type, the ookinete. DOZI and CITH are mRNP components that maintain mRNAs silent in the female precursor cell. In the absence of DOZI or CITH, hundreds of transcripts are destabilised, which does not block fertilization upon transmission to the mosquito host, but impairs further development of the malaria parasite. However, how many transcripts are bound by DOZI/CITH in these mRNPs and what are they remain unknown. Here, we investigated the extent of maternal contribution during post-fertilization development of P. berghei by RNA immunoprecipitation (RIP)-Chip data mining and provided further functional significance using gene tagging and gene knockout approaches. We provide conclusive evidence that in the rodent malaria parasite Plasmodium berghei 50% of the transcriptome of the female sexual precursor cell is stably associated with the translational repressors DOZI and CITH allowing morphogenesis of the zygote into the ookinete to proceed in the absence of RNA polymerase II transcription. Using in situ tagging with GFP we find for the first time novel genes that rely on translational repression on the 5' UTR. This maternal repressome provides the developing ookinete with coding potential for key molecules required during developmental progression: a near complete set of factors required for the gliding motility apparatus, but also protein trafficking, adhesins, proteases and other factors.

2014-10-23 | E-MTAB-2900 | biostudies-arrayexpress

Plasmodium berghei WT and ap2-o disruptant ookinete culture 12h

Project description:Plasmodium berghei WT and ap2-o disruptant ookinete culture 12h Plasmodium berghei WT and ap2-o disruptant ookinete culture 12h

2010-05-14 | E-GEOD-14376 | biostudies-arrayexpress

Identification of AP2-O targets [ChIP-seq II]

Project description:Stage-specific transcription is a fundamental biological process in the lifecycle of the Plasmodium parasite. Proteins containing the AP2 DNA-binding domain are responsible for stage-specific transcriptional regulation, and belong to the only known family of transcription factors in Plasmodium parasites. A comprehensive identification of their target genes will advance our understanding of the molecular basis of stage-specific parasite development. In this study, we have used ChIP-seq technology to identify the target genes of the ookinete-specific AP2 transcription factor AP2-O.

2015-06-01 | GSE58583 | GEO

Identification of AP2-O targets [ChIP-seq I]

2015-06-01 | GSE58582 | GEO

Identification of AP2-O targets [RNA-Seq]

2015-06-01 | GSE58581 | GEO

Identification of AP2-O targets [microarray]

2015-06-01 | GSE58580 | GEO

Cdc14 as transcription regulator of RNA pol-II

Project description:In yeast, the phosphatase Cdc14 is required for mitotic exit and for segregation of repetitive regions. Cdc14 is also a subunit of the silencing complex RENT, but no roles in transcription repression have been described. Here we report that Cdc14 promotes silencing at the integenic spacer sequences (IGS) of ribosomal genes during interphase and at Y' repeats in sub-telomeric regions during mitosis, through its ability to dephosphotylate the CDT domain of the RNA polymerase II. CDC14 and CDC15 strains were grown at 25Â°C and 37Â°C. Comparisons were made between each strain at different temperatures, and between strains at the same temperature.

2011-07-28 | E-GEOD-31020 | biostudies-arrayexpress

Cdc14 as transcription regulator of RNA pol-II

2011-07-29 | GSE31020 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data